Design and synthesis of a novel fluorescent peptide substrate of SARS-CoV 3CL(pro) and studies on its enzymatic kinetics
文献类型：期刊论文
| 作者 | Wan Hui-Xin; Chen Li-Li ; Li Xin; Wang Xin; Hu Ding-Yu; Shen Xu; Shen Jing-Kang
|
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2007-01-10 |
| 卷号 | 28期号:1页码:79-82 |
| 关键词 | fluorescence resonance energy transfer(FRET) SARS-CoV 3CL(pro) fluorescent probe LC-MS |
| ISSN号 | 0251-0790 |
| 文献子类 | Article |
| 英文摘要 | A novel fluorescent peptide substrate, H-2 N-E (Eddnp) STLQSGLK (Abz)-CONH2, based on fluorescence resonance energy transfer(FRET) technique, was designed and synthesized by solid-phase peptide synthesis. Abz/Eddnp was used as the fluorescence donor and quenching pair. The highly fluorescent Abz group was efficiently quenched by energy transfer to the Eddnp group. The fluorescent substrate was specifically hydrolyzed by SARS-CoV 3CL(pro) between QS with 15-fold increase in fluorescence and the cleavage site was determined by HPLC-MS. The studies on its enzymatic kinetics(K-m = 525.5 mu mol/L and K-cat = 1.29 min(-1)) elucidate that the synthetic fluorescent peptide substrate is suited to be used as a fluorescent probe in determination of the activity of SARS-CoV 3CL(pro) and screening assays for its inhibitors. |
| WOS关键词 | ACUTE RESPIRATORY SYNDROME ; RESONANCE ENERGY-TRANSFER ; 3C-LIKE PROTEINASE ; SYNDROME CORONAVIRUS ; PROTEASE ; PURIFICATION ; INHIBITOR ; PROBES ; ASSAY |
| WOS研究方向 | Chemistry |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2734733 |
| WOS记录号 | WOS:000243581800019 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273358]  |
| 专题 | 药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Shen Jing-Kang |
| 作者单位 | Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan Hui-Xin,Chen Li-Li,Li Xin,et al. Design and synthesis of a novel fluorescent peptide substrate of SARS-CoV 3CL(pro) and studies on its enzymatic kinetics[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(1):79-82. |
| APA | Wan Hui-Xin.,Chen Li-Li.,Li Xin.,Wang Xin.,Hu Ding-Yu.,...&Shen Jing-Kang.(2007).Design and synthesis of a novel fluorescent peptide substrate of SARS-CoV 3CL(pro) and studies on its enzymatic kinetics.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(1),79-82. |
| MLA | Wan Hui-Xin,et al."Design and synthesis of a novel fluorescent peptide substrate of SARS-CoV 3CL(pro) and studies on its enzymatic kinetics".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.1(2007):79-82. |
入库方式： OAI收割
来源：上海药物研究所
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