Grateloupia longifolia polysaccharide inhibits angiogenesis by downregulating tissue factor expression in HMEC-1 endothelial cells
文献类型：期刊论文
| 作者 | Zhang, Chao; Yang, Fan; Zhang, Xiong-Wen; Wang, Shun-Chun; Li, Mei-Hong; Lin, Li-Ping; Ding, Jian
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| 刊名 | BRITISH JOURNAL OF PHARMACOLOGY
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| 出版日期 | 2006-07 |
| 卷号 | 148期号:6页码:741-751 |
| 关键词 | Grateloupia longifolia polysaccharide antiangiogenesis tissue factor HMEC-1 |
| ISSN号 | 0007-1188 |
| DOI | 10.1038/sj.bjp.0706741 |
| 文献子类 | Article |
| 英文摘要 | 1 The antiangiogenic and antitumor properties of Grateloupia longifolia polysaccharide (GLP), a new type of polysaccharide isolated from the marine alga, were investigated with several in vitro and in vivo models. Possible mechanisms underlying its antiangiogenic activity were also assessed. 2 GLP dose-dependently inhibited proliferation of human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC), with IC50 values of 0.86 and 0.64 mg ml(-1), respectively. In tube formation and cell migration assays using HMEC-1 cells, noncytotoxic doses of GLP significantly inhibited formation of intact tube networks and reduced the number of migratory cells. Inhibition by GLP was VEGF-independent. 3 In the chick chorioallantoic membrane (CAM) assay, GLP (2.5 mu g egg(-1)) reduced new vessel formation compared with the vehicle control. GLP (0.1 mg plug(-1)) also reduced the vessel density in Matrigel plugs implanted in mice. 4 The levels of pan and phosphorylated recptors for VEGF, VEGFR-1 (flt-1) and VEGFR-2 (KDR) were not significantly altered by 5 mg ml(-1) GLP treatment of HMEC-1, although tissue factor (TF) showed significant decreases at both mRNA and protein levels following GLP treatment. 5 In mice bearing sarcoma-180 cells, intravenous administration of GLP (200 mg kg(-1)) decreased tumor weight by 52% without obvious toxicity. Vascular density in sections of the tumor was reduced by 64% after GLP treatment. 6 Collectively, these results indicate that GLP has antitumor properties, associated at least, in part, with the antiangiogenesis induced by downregulation of TF. |
| WOS关键词 | PENTOSAN POLYSULFATE ; TUMOR-GROWTH ; ANTITUMOR ; NEOVASCULARIZATION ; METASTASIS ; CANCER |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000239385900001 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273547]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhang, Xiong-Wen |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Div Anti Tumor Pharmacol, Shanghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China 3.Shanghai Univ Tradit Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chao,Yang, Fan,Zhang, Xiong-Wen,et al. Grateloupia longifolia polysaccharide inhibits angiogenesis by downregulating tissue factor expression in HMEC-1 endothelial cells[J]. BRITISH JOURNAL OF PHARMACOLOGY,2006,148(6):741-751. |
| APA | Zhang, Chao.,Yang, Fan.,Zhang, Xiong-Wen.,Wang, Shun-Chun.,Li, Mei-Hong.,...&Ding, Jian.(2006).Grateloupia longifolia polysaccharide inhibits angiogenesis by downregulating tissue factor expression in HMEC-1 endothelial cells.BRITISH JOURNAL OF PHARMACOLOGY,148(6),741-751. |
| MLA | Zhang, Chao,et al."Grateloupia longifolia polysaccharide inhibits angiogenesis by downregulating tissue factor expression in HMEC-1 endothelial cells".BRITISH JOURNAL OF PHARMACOLOGY 148.6(2006):741-751. |
入库方式： OAI收割
来源：上海药物研究所
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