Pseudolarix acid B, a new tubulin-binding agent, inhibits angiogenesis by interacting with a novel binding site on tubulin
文献类型：期刊论文
| 作者 | Tong, YG; Zhang, XW; Geng, MY ; Yue, JM; Xin, XL; Tian, F; Shen, X; Tong, LJ; Li, MH; Zhang, C
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| 刊名 | MOLECULAR PHARMACOLOGY
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| 出版日期 | 2006-04 |
| 卷号 | 69期号:4页码:1226-1233 |
| ISSN号 | 0026-895X |
| DOI | 10.1124/mol.105.020537 |
| 文献子类 | Article |
| 英文摘要 | Tubulin-binding agents have received considerable interest as potential tumor-selective angiogenesis-targeting drugs. Herein, we report that pseudolarix acid B (PAB), isolated from the traditional Chinese medicinal plant Pseudolarix kaempferi Gordon, is a tubulin-binding agent. We further demonstrate that PAB significantly and dose-dependently inhibits proliferation, migration, and tube formation by human microvessel enthothelial cells. It is noteworthy that PAB eliminated newly formed endothelial tubes and microvessels both in vitro and in vivo. In addition, PAB dramatically arrested the cell cycle at G(2)/M phase. PAB also induced endothelial cell retraction, intercellular gap formation, and promoted actin stress fiber formation in conjunction with disruption of the tubulin and actin cytoskeletons. All of these effects occurred at noncytotoxic concentrations of PAB. We found that these effects of PAB are attributable to depolymerization of tubulin by direct interaction with a distinct binding site on tubulin compared with those of colchicine and vinblastine. Taken together, these findings show that PAB is a candidate antiangiogenic agent for use in cancer therapy, and they provide proof of principle for targeting this novel binding site on tubulin as a new strategy for treating cancer. |
| WOS关键词 | TUBE FORMATION ; IN-VITRO ; CYTOSKELETON ; MICROTUBULE ; COLCHICINE ; FLUORESCENCE ; VINBLASTINE ; MECHANISM ; MIGRATION ; TARGET |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000236162600017 |
| 出版者 | AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273635]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, J |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai Inst Mat Med,Div Antitumor Pharmacol, Shanghai 201203, Peoples R China 2.Ocean Univ China, Dept Pharmacol, Marine Drug & Food Inst, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tong, YG,Zhang, XW,Geng, MY,et al. Pseudolarix acid B, a new tubulin-binding agent, inhibits angiogenesis by interacting with a novel binding site on tubulin[J]. MOLECULAR PHARMACOLOGY,2006,69(4):1226-1233. |
| APA | Tong, YG.,Zhang, XW.,Geng, MY.,Yue, JM.,Xin, XL.,...&Ding, J.(2006).Pseudolarix acid B, a new tubulin-binding agent, inhibits angiogenesis by interacting with a novel binding site on tubulin.MOLECULAR PHARMACOLOGY,69(4),1226-1233. |
| MLA | Tong, YG,et al."Pseudolarix acid B, a new tubulin-binding agent, inhibits angiogenesis by interacting with a novel binding site on tubulin".MOLECULAR PHARMACOLOGY 69.4(2006):1226-1233. |
入库方式： OAI收割
来源：上海药物研究所
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