Suppression of (5R)-5-hydroxytriptolide (LLDT-8) on allograft rejection in full MHC-mismatched mouse cardiac transplantation
文献类型：期刊论文
| 作者 | Tang, W ; Zhou, R; Yang, Y; Li, YC; Yang, YF; Zuo, JP
|
| 刊名 | TRANSPLANTATION
 |
| 出版日期 | 2006-03-27 |
| 卷号 | 81期号:6页码:927-933 |
| ISSN号 | 0041-1337 |
| 关键词 | (5R)-5-hydroxytriptolide cardiac transplantation chemokine immunosuppression |
| DOI | 10.1097/01.tp.0000203299.39843.d2 |
| 文献子类 | Article |
| 英文摘要 | Background. (5R)-5-hydroxytriptolide (LLDT-8) is a new Compound derived from triptolide, which is the major immunosuppressive fraction of Tripterygium wilfordii Hook. F(TWHF). Studies in vitro and in vivo have demonstrated that LLDT-8 had potent immunosuppressive activities. Here we tested LLDT-8 in major histocompatibility complex (MHC)-mismatched cardiac transplantation and investigated the mechanisms underlying the prevention of transplant rejection. Methods. LLDT-8 was administered orally to recipients in Balb/c to C57BL/6 murine cardiac transplantation model. Allograft survival after transplantation was recorded in recipients. The T cell immunity and cytokine production were observed. Histological analysis was performed. The chemokine and its receptor were analyzed by reverse transcriptase-polymerase chain reaction on cardiac graft RNA. Results. LLDT-8 administered orally significantly induced the survival prolongation of allogeneic cardiac graft. Histological results showed that LLDT-8 well preserved myocardium and significantly reduced infiltration of the graft with inflammatory cells. LLDT-8 decreased IL-2 production in recipient splenocytes stimulated by concanavalin A (ConA) ex vivo. LLDT-8 significantly inhibited the immunoreactivity of recipient to specific donor alloantigens, but preserved immunity to third-part), alloantigens and mitogen. However, the flow cytometry analysis of the proportion of CD4(+), CD8(+) T cell subgroup in recipient spleens showed LLDT-8 had a normalizing effect on the splenic lymphocytes Population. LLDT-8 decreased CC chemokine receptor 5 (CCR5) and their ligands macrophage inflammatory protein 1 alpha (MIP-1 alpha) and beta (MIP-1 beta) mRNA expressions in allografts. Conclusion. The results Outline the great potential of LLDT-8 as a therapeutic tool in transplant rejection. |
| WOS关键词 | WILFORDII HOOK F ; RECEPTOR-GENE-EXPRESSION ; VERSUS-HOST-DISEASE ; TRIPTERYGIUM-WILFORDII ; LYMPHOPROLIFERATIVE DISORDERS ; RENAL-TRANSPLANTATION ; ORGAN-TRANSPLANTATION ; PERIPHERAL TOLERANCE ; T-CELLS ; TRIPTOLIDE |
| WOS研究方向 | Immunology ; Surgery ; Transplantation |
| 语种 | 英语 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| WOS记录号 | WOS:000236440900019 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273640]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zuo, JP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol,Grad Sch, Shanghai Inst Biol Sci,State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Shanghai Univ, Lab Immunol & Virol, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Lab, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, W,Zhou, R,Yang, Y,et al. Suppression of (5R)-5-hydroxytriptolide (LLDT-8) on allograft rejection in full MHC-mismatched mouse cardiac transplantation[J]. TRANSPLANTATION,2006,81(6):927-933. |
| APA | Tang, W,Zhou, R,Yang, Y,Li, YC,Yang, YF,&Zuo, JP.(2006).Suppression of (5R)-5-hydroxytriptolide (LLDT-8) on allograft rejection in full MHC-mismatched mouse cardiac transplantation.TRANSPLANTATION,81(6),927-933. |
| MLA | Tang, W,et al."Suppression of (5R)-5-hydroxytriptolide (LLDT-8) on allograft rejection in full MHC-mismatched mouse cardiac transplantation".TRANSPLANTATION 81.6(2006):927-933. |
入库方式： OAI收割
来源：上海药物研究所
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