Induction of liver cytochrome P450 1A2 expression by flutamide in rats
文献类型：期刊论文
| 作者 | Wang, HX; Liu, X ; Xu, CJ; Ma, XC; Long, JE; Li, D
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2005-11 |
| 卷号 | 26期号:11页码:1382-1386 |
| 关键词 | flutamide cytochrome P450 1A2 induction |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2005.00211.x |
| 文献子类 | Article |
| 英文摘要 | Aim: To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Methods: Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP 1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate. Results: CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity. Conclusion: Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner. |
| WOS关键词 | NONSTEROIDAL ANTIANDROGEN ; SCH 13521 ; METABOLISM ; THERAPY ; HEPATOTOXICITY ; CAFFEINE ; FAILURE ; ENZYME |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2165409 |
| WOS记录号 | WOS:000233209800016 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273775]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Li, D |
| 作者单位 | 1.Fudan Univ, Sch Basic Med Sci, Inst Mol Virus, Shanghai 200031, Peoples R China 2.Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Inst Biol Sci, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, HX,Liu, X,Xu, CJ,et al. Induction of liver cytochrome P450 1A2 expression by flutamide in rats[J]. ACTA PHARMACOLOGICA SINICA,2005,26(11):1382-1386. |
| APA | Wang, HX,Liu, X,Xu, CJ,Ma, XC,Long, JE,&Li, D.(2005).Induction of liver cytochrome P450 1A2 expression by flutamide in rats.ACTA PHARMACOLOGICA SINICA,26(11),1382-1386. |
| MLA | Wang, HX,et al."Induction of liver cytochrome P450 1A2 expression by flutamide in rats".ACTA PHARMACOLOGICA SINICA 26.11(2005):1382-1386. |
入库方式： OAI收割
来源：上海药物研究所
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