Huperzine A protects SHSY5Y neuroblastoma cells against oxidative stress damage via nerve growth factor production
文献类型：期刊论文
| 作者 | Tang, LL; Wang, R; Tang, XC
|
| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2005-09-05 |
| 卷号 | 519期号:1-2页码:9-15 |
| 关键词 | huperzine A oxidative stress nerve growth factor MAP (mitogen-activated protein) kinase TrkA (tyrosine kinase A) receptor |
| ISSN号 | 0014-2999 |
| DOI | 10.1016/j.ejphar.2005.06.026 |
| 文献子类 | Article |
| 英文摘要 | Our previous study demonstrated that huperzine A, a selective acetylcholinesterase inhibitor, stimulates the synthesis of nerve growth factor (NGF) in cultured rat cortical astrocytes. The present studies are designed to examine if huperzine A exerts its neuroprotective activity against oxidative stress damage through increasing the synthesis of NGF in SHSY5Y neuroblastoma cells. Transient exposure of the cells to 200 mu M H2O2 triggered a significant reduction of cell viability and decreased the mRNA and protein levels of NGF, neurotrophin receptor P75 (P75(NTR)) receptor and tyrosine kinase A (TrkA) receptor. Incubation of cells with 10 mu M huperzine A prior to H2O2 exposure significantly elevated their survival and restored the mRNA and protein levels of NGF, P75(NTR) receptor and TrkA receptor. These neuroprotective effects of huperzine A on H2O2-induced cytotoxicity were blocked by the TrkA receptor phosphorylation inhibitor K252 alpha, and were antagonized by the mitogen-activated protein (MAP)/extracellular signal-regulated kinase (ERK) inhibitor, PD98059. The present results indicate that the cytoprotective effect of huperzine A is mediated at least partly by up-regulated NGF and NGF receptors. The results also show that the MAP/ERK kinase signal pathway is crucial for huperzine A to protect against H2O2-induced damage in SHSY5Y cells. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS关键词 | OXIDANT-ANTIOXIDANT BALANCE ; RAT PC12 CELLS ; NEUROTROPHIN RECEPTORS ; GLUTATHIONE-PEROXIDASE ; ALZHEIMERS-DISEASE ; NEURITE OUTGROWTH ; NEURONAL INJURY ; SURVIVAL ; NGF ; ACTIVATION |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000231847100002 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273805]  |
| 专题 | 院士及顾问专家 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Tang, XC |
| 作者单位 | Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, LL,Wang, R,Tang, XC. Huperzine A protects SHSY5Y neuroblastoma cells against oxidative stress damage via nerve growth factor production[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2005,519(1-2):9-15. |
| APA | Tang, LL,Wang, R,&Tang, XC.(2005).Huperzine A protects SHSY5Y neuroblastoma cells against oxidative stress damage via nerve growth factor production.EUROPEAN JOURNAL OF PHARMACOLOGY,519(1-2),9-15. |
| MLA | Tang, LL,et al."Huperzine A protects SHSY5Y neuroblastoma cells against oxidative stress damage via nerve growth factor production".EUROPEAN JOURNAL OF PHARMACOLOGY 519.1-2(2005):9-15. |
入库方式： OAI收割
来源：上海药物研究所
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