A validated stability-indicating HPLC with photodiode array detector (PDA) method for the stress tests of Monascus purpureus-fermented rice, red yeast rice
文献类型：期刊论文
| 作者 | Li, YG; Liu, H ; Wang, ZT
|
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 39期号:1-2页码:82-90 |
| 关键词 | stability-indicating assay method (SIAM) stress tests Monascus purpureus monacolins |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2005.03.005 |
| 文献子类 | Article |
| 英文摘要 | A stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detection method was developed and validated for the assay of monacolin series compounds including monacolin K, L, J and their hydroxyl acid forms as well as dehydroxymonacolin K simultaneously in Monascus purpureus-fermented rice, red yeast rice. Well-resolved peaks of seven main compounds of monacolin family were profiled on a C-18 reverse-phase column using a linear gradient of 0.1% trifluoroacetic acid and acetonitrile as the mobile phase, and the detection wavelength was set at 237 nm. The method was validated with respect to specificity, chromatographic parameters, linearity, precision, accuracy, limits of detection and quantitation. The stability stress testing for fermented red yeast rice powder was carried out to show the effects of high temperature (80 degrees C), high humidity at room temperature (92.5 % RH, 25 degrees C), high humidity at high temperature (75% RH, 60 degrees C) and light (sunlight) in solid state. The results exhibited that monacolins decreased significantly under the conditions of high humidity at high temperature (75% RH, 60 degrees C) and sunlight. Monacolin K and its hydroxyl acid form would be dehydrolyzed and turned to dehydromonacolin K at high temperature (80 degrees C) while the monacolin K, J and L would be transformed into their corresponding hydroxyl acid forms under the condition of high humidity (92.5% RH, 25 degrees C). The indication is that monacolins in red yeast rice powder are light-sensitive and thermal-sensitive. Therefore, it has been suggested that the preparations containing monacolins be stored in the place of cool and lightproof. The proposed degradation pathways were discussed as well. The multi-components assay for stability of botanical products could provide much more information than the normal marker-orientation method. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; METABOLITES ; CHOLESTEROL ; MEDICINE ; PRODUCT ; ASSAY ; FOOD ; DRUG |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000232209600013 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273810]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Li, YG |
| 作者单位 | 1.Shanghai Univ Trad Chinese Med, Shanghai R&D Ctr Standardizat Trad Chinese Med, Shanghai 201203, Peoples R China 2.Shanghai Univ Trad Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Ctr Drug Discovery & Design, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, YG,Liu, H,Wang, ZT. A validated stability-indicating HPLC with photodiode array detector (PDA) method for the stress tests of Monascus purpureus-fermented rice, red yeast rice[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,39(1-2):82-90. |
| APA | Li, YG,Liu, H,&Wang, ZT.(2005).A validated stability-indicating HPLC with photodiode array detector (PDA) method for the stress tests of Monascus purpureus-fermented rice, red yeast rice.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,39(1-2),82-90. |
| MLA | Li, YG,et al."A validated stability-indicating HPLC with photodiode array detector (PDA) method for the stress tests of Monascus purpureus-fermented rice, red yeast rice".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 39.1-2(2005):82-90. |
入库方式： OAI收割
来源：上海药物研究所
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