Effects of 5 daily injections of the neurotensin-mimetic NT69L on the expression of neurotensin receptors in rat brain
文献类型：期刊论文
| 作者 | Wang, R; Boules, M; Gollatz, E; Williams, K; Tiner, W; Richelson, E |
| 刊名 | MOLECULAR BRAIN RESEARCH
 |
| 出版日期 | 2005-07-29 |
| 卷号 | 138期号:1页码:24-34 |
| 关键词 | neurotensin agonist tolerance neurotensin receptors mRNA tyrosine hydroxylase rat |
| ISSN号 | 0169-328X |
| DOI | 10.1016/j.molbrainres.2005.03.014 |
| 文献子类 | Article |
| 英文摘要 | The effects of one or five daily intraperitoneal injections of a neurotensin (NT) receptor agonist NT69L (2 mg/kg, i.p.) on the expression of NT (NTS), dopamine I and 2 receptors, tyrosine hydroxylase, and DOPA decarboxylase using immunohistochemical and real-time PCR were investigated in rats. Except for the striatum, acute injection of NT69L did not affect neurotensin receptors as compared to saline control. However, 5 daily injections of NT69L resulted in down-regulation of both NTS-I protein and mRNA levels in several brain regions with the striatum showing a dramatic decrease in NTS-1 expression (P < 0.05). The down-regulation of NTS-1 in the striaturn, hypothalamus, and substania nigra (SN) after 5 daily injections was confirmed by autoradiography. Acute injection of NT69L. increased NTS-2 mRNA and protein level in prefrontal cortex (PFC). NTS-3 mRNA expression and protein levels were slightly down-regulated in hypothalamus. periaqueductal gray (PAG), and SN, though the difference was not significant. The results indicated a difference in the profile of NT receptors expression in response to NT69L.. Tyrosine hydroxylase (TH) and DOPA deearboxylase (DE)Q mRNA was significantly down-regulated in striatum but not in SN. Interestingly, Nurrl, a transcriptional activator of TH, was dramatically up-regulated in striatum. but down-regulated in PFC, suggesting that different modulating mechanisms may participate in NT69L tolerance in different regions, The present results suggest that distinct NT receptors involved in the effects exerted by NT69L may contribute to the interactions of NT69L with both neural networks and cellular proteins. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS关键词 | MESSENGER-RNA ; PEPTIDE ANALOG ; MECHANISMS ; ANTINOCICEPTION ; HYPOTHERMIA ; RESPONSES ; BARRIER ; NEURONS ; DESENSITIZATION ; ACTIVATION |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231205600004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273831]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Boules, M |
| 作者单位 | 1.Mayo Clin Jacksonville, Jacksonville, FL 32224 USA 2.Mayo Fdn Med Educ & Res, Neuropsychopharmacol Lab, Jacksonville, FL 32224 USA 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, R,Boules, M,Gollatz, E,et al. Effects of 5 daily injections of the neurotensin-mimetic NT69L on the expression of neurotensin receptors in rat brain[J]. MOLECULAR BRAIN RESEARCH,2005,138(1):24-34. |
| APA | Wang, R,Boules, M,Gollatz, E,Williams, K,Tiner, W,&Richelson, E.(2005).Effects of 5 daily injections of the neurotensin-mimetic NT69L on the expression of neurotensin receptors in rat brain.MOLECULAR BRAIN RESEARCH,138(1),24-34. |
| MLA | Wang, R,et al."Effects of 5 daily injections of the neurotensin-mimetic NT69L on the expression of neurotensin receptors in rat brain".MOLECULAR BRAIN RESEARCH 138.1(2005):24-34. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。