Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells
文献类型：期刊论文
| 作者 | Liu, J ; Wu, QL; Feng, YH; Yang, YF; Li, XY; Zuo, JP
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2005-02 |
| 卷号 | 26期号:2页码:223-227 |
| 关键词 | triptolide CD80 antigens CD86 antigens interleukin-12 |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2005.00035.x |
| 文献子类 | Article |
| 英文摘要 | Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expression and interleukin-12 (IL-12) production from THP-1 cells. Methods: THP-1 cells were differentiated into macrophage-like cells by Me(2)SO, and then cultured with IFN-gamma (500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL-12p40, IL-12p70 were assayed by ELISA. Results: Tripolide suppressed CD80 and CD86 expressions on IFN-gamma (500 kU/L) and LPS (1 mg/L) activated THP-1 cells at nontoxic dosages of 2.5-0.625 mug/L. Furthermore, the production of IL-12p40 and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide. Conclusion: Triptolide impairs the antigen-presenting function by inhibiting CD80 and CD86 expressions and decreased IL-12p40 and IL-12p70 (bioactive form) productions from the activated THP-1 cells. |
| WOS关键词 | NF-KAPPA-B ; TUBULAR EPITHELIAL-CELLS ; BLOOD MONONUCLEAR-CELLS ; RHEUMATOID-ARTHRITIS ; MOLECULES ; IMMUNOSUPPRESSANT ; INTERLEUKIN-12 ; PROLIFERATION ; COSTIMULATION ; INHIBITION |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1918447 |
| WOS记录号 | WOS:000226942000016 |
| 出版者 | SHANGHAI INST MATERIA MEDICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273914]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物发现与设计中心 分析化学研究室 上海药物代谢研究中心 |
| 通讯作者 | Zuo, JP |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, J,Wu, QL,Feng, YH,et al. Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells[J]. ACTA PHARMACOLOGICA SINICA,2005,26(2):223-227. |
| APA | Liu, J,Wu, QL,Feng, YH,Yang, YF,Li, XY,&Zuo, JP.(2005).Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells.ACTA PHARMACOLOGICA SINICA,26(2),223-227. |
| MLA | Liu, J,et al."Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells".ACTA PHARMACOLOGICA SINICA 26.2(2005):223-227. |
入库方式： OAI收割
来源：上海药物研究所
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