Immunogenic comparison of two coupling methods of marine polysaccharide to bovine serum albumin
文献类型：期刊论文
| 作者 | Li, G; Xin, XL; Geng, MY ; Ding, J
|
| 刊名 | BIOLOGICAL & PHARMACEUTICAL BULLETIN
 |
| 出版日期 | 2004-10 |
| 卷号 | 27期号:10页码:1532-1536 |
| 关键词 | marine polysaccharide monoclonal antibody periodate oxidation reductive amination |
| ISSN号 | 0918-6158 |
| DOI | 10.1248/bpb.27.1532 |
| 文献子类 | Article |
| 英文摘要 | Two conjugates of marine polysaccharide (MPS) and bovine serum albumin (BSA) were prepared using two methods, periodate oxidation and reductive amination, with the intent of enhancing its immunogenicity. Sera samples from Balb/c mice immunized with the products named MPS-BSAp and MPS-BSAr respectively were evaluated by enzyme-linked-immunosorbent assay (ELISA). The results showed that mice immunized with MPS-BSAp produced antibodies not against MPS but rather against MPS-BSAp, while the mice immunized with MPS-BSAr produced high titer antibodies only specific for MPS. The difference was attributed to the fact that the epitopes of MPS had been changed in the coupling process by periodate oxidation. A mouse immunized with MPS-BSAr was chosen to prepare monoclonal antibodies (mAbs) specific for polysaccharide MPS. A bybridoma cell line that secreted monoclonal antibody recognizing specifically polysaccharide MPS was established. |
| WOS关键词 | TOXOID CONJUGATE VACCINE ; GROUP-B STREPTOCOCCUS ; MONOCLONAL-ANTIBODIES ; SPECIFICALLY RECOGNIZES ; PEPTIDE ANTIGENS ; HEPARIN ; ELISA ; SULFATE ; IDENTIFICATION ; CHAIN |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000224522100009 |
| 出版者 | PHARMACEUTICAL SOC JAPAN |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274008]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Geng, MY |
| 作者单位 | 1.Ocean Univ China, Dept Pharmacol, Marine Drug & Food Inst, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Div Antitumor Pharmacol,State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, G,Xin, XL,Geng, MY,et al. Immunogenic comparison of two coupling methods of marine polysaccharide to bovine serum albumin[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2004,27(10):1532-1536. |
| APA | Li, G,Xin, XL,Geng, MY,&Ding, J.(2004).Immunogenic comparison of two coupling methods of marine polysaccharide to bovine serum albumin.BIOLOGICAL & PHARMACEUTICAL BULLETIN,27(10),1532-1536. |
| MLA | Li, G,et al."Immunogenic comparison of two coupling methods of marine polysaccharide to bovine serum albumin".BIOLOGICAL & PHARMACEUTICAL BULLETIN 27.10(2004):1532-1536. |
入库方式： OAI收割
来源：上海药物研究所
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