Alternative splicing of human telomerase reverse transcriptase may not be involved in telomerase regulation during all-trans-retinoic acid-induced HL-60 cell differentiation
文献类型：期刊论文
| 作者 | Liu, WJ; Zhang, YW; Zhang, ZX; Jian, D |
| 刊名 | JOURNAL OF PHARMACOLOGICAL SCIENCES
 |
| 出版日期 | 2004-10 |
| 卷号 | 96期号:2页码:106-114 |
| 关键词 | telomerase human telomerase reverse transcriptase alternative splicing differentiation all-trans-retinoic acid |
| ISSN号 | 1347-8613 |
| DOI | 10.1254/jphs.FP0030600 |
| 文献子类 | Article |
| 英文摘要 | Alternative splicing of the human telomerase reverse transcriptase subunit (hTERT) suppresses telomerase activity during the development of human fetal kidney cells into mature cells. Tumor cell differentiation is the process of turning abnormal tumor cells into 'normal' cells accompanied by down-regulation of telomerase activity. However, the precise mechanism of the regulation of telomerase activity in differentiated cells is not fully understood. In this study, we observed the role of alternative splicing of hTERT in the regulation of telomerase activity in all-trans-retinoic acid (ATRA)-induced, differentiated HL-60 cells. ATRA-induced downregulation of telomerase activity in differentiated HL-60 cells was associated with a decrease in hTERT and an increase in human telomerase-associated protein-1 (hTP1) transcription. Expression of full length variant hTERT alpha(+)beta(+) mRNA decreased in a dose- and time-dependent manner. The drop of hTERT beta(-) mRNA was time-dependent. hTERT alpha(-) and hTERT alpha(-)beta(-) mRNA were reduced dramatically after ATRA treatment. In the dose-effect study, hTERT alpha(+)beta(+) and hTERT beta(-) maintained a relatively stable ratio when telomerase activity decreased largely from treatment with I to 5 muM ATRA. Although the splicing pattern of hTERT mRNA was altered in time-effect research, the change was not related to the ATRA-treated decline of telomerase activity. The expression of alternative splicing variants of hTERT also decreased at the protein level. All these results suggested that alternative splicing of hTERT mRNA may not contribute to the suppression of telomerase activity during ATRA-induced HL-60 leukemia cell differentiation. |
| WOS关键词 | CATALYTIC SUBUNIT ; DOWN-REGULATION ; HEMATOPOIETIC-CELLS ; IMMORTAL CELLS ; HL60 CELLS ; IN-VITRO ; HTERT ; PROTEIN ; EXPRESSION ; GENE |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000224694100003 |
| 出版者 | JAPANESE PHARMACOLOGICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274014]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Jian, D |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol,State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, WJ,Zhang, YW,Zhang, ZX,et al. Alternative splicing of human telomerase reverse transcriptase may not be involved in telomerase regulation during all-trans-retinoic acid-induced HL-60 cell differentiation[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2004,96(2):106-114. |
| APA | Liu, WJ,Zhang, YW,Zhang, ZX,&Jian, D.(2004).Alternative splicing of human telomerase reverse transcriptase may not be involved in telomerase regulation during all-trans-retinoic acid-induced HL-60 cell differentiation.JOURNAL OF PHARMACOLOGICAL SCIENCES,96(2),106-114. |
| MLA | Liu, WJ,et al."Alternative splicing of human telomerase reverse transcriptase may not be involved in telomerase regulation during all-trans-retinoic acid-induced HL-60 cell differentiation".JOURNAL OF PHARMACOLOGICAL SCIENCES 96.2(2004):106-114. |
入库方式： OAI收割
来源：上海药物研究所
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