ER beta exerts multiple stimulative effects on human breast carcinoma cells
文献类型:期刊论文
作者 | Hou, YF; Yuan, ST; Li, HC; Wu, J; Lu, JS; Liu, G; Lu, LJ; Shen, ZZ; Ding, J![]() |
刊名 | ONCOGENE
![]() |
出版日期 | 2004-07-29 |
卷号 | 23期号:34页码:5799-5806 |
关键词 | breast carcinoma estrogen receptor matrix metalloproteinases (MMPs) |
ISSN号 | 0950-9232 |
DOI | 10.1038/sj.onc.1207765 |
文献子类 | Article |
英文摘要 | Recent studies of ERs in breast cancer have demonstrated the existence of ERbeta in addition to ERalpha. Some clinical data indicated that ERbeta had prognostic value for patient's survival, which suggested that ERbeta plays a key role in breast cancer development and,metastasis. To test this hypothesis, we generated an ERbeta high-expression cell line by reintroduced human ERbeta cDNA into MDA-MB-435 cells. We demonstrated that ERbeta exerted multiple tumor-stimulative effects on human breast carcinoma cells both in vivo and in vitro. In in vitro studies, ERbeta was able to increase the proliferation and invasion of MDA-MB-435 cells significantly, while these effects were totally estradiol independent. Also, this stimulation was characterized by downregulation of p2l and upregulation of MMP-9, as well as transcriptional factor Est-1. In in vivo studies, we also demonstrated that ERbeta-transfected MDA-MB-435 cells grew much faster and had more pulmonary metastasis than mock or wild-type cells in nude mice. In ERbeta-transfected MDA-MB-435 xenografts, ERbeta caused significant reduction in p2l protein levels. Similar effects of ERbeta on MMP-9 and Ets-1 expression noted in vitro studies were also observed in the in vivo studies. These in vitro and in vivo studies indicated that ERbeta exerted multiple stimulative effects on breast cancer development and metastasis. |
WOS关键词 | ESTROGEN-RECEPTOR-BETA ; MESSENGER-RNA EXPRESSION ; MATRIX METALLOPROTEINASE-2 ; ENDOMETRIAL CARCINOMA ; METASTATIC PHENOTYPE ; CANCER ; ALPHA ; GENE ; IDENTIFICATION ; PROLIFERATION |
WOS研究方向 | Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity |
语种 | 英语 |
WOS记录号 | WOS:000222629800009 |
出版者 | NATURE PUBLISHING GROUP |
源URL | [http://119.78.100.183/handle/2S10ELR8/274044] ![]() |
专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
通讯作者 | Ding, J |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Fudan Univ, Dept Surg, Canc Hosp, Inst Canc, Shanghai 200032, Peoples R China |
推荐引用方式 GB/T 7714 | Hou, YF,Yuan, ST,Li, HC,et al. ER beta exerts multiple stimulative effects on human breast carcinoma cells[J]. ONCOGENE,2004,23(34):5799-5806. |
APA | Hou, YF.,Yuan, ST.,Li, HC.,Wu, J.,Lu, JS.,...&Shao, ZM.(2004).ER beta exerts multiple stimulative effects on human breast carcinoma cells.ONCOGENE,23(34),5799-5806. |
MLA | Hou, YF,et al."ER beta exerts multiple stimulative effects on human breast carcinoma cells".ONCOGENE 23.34(2004):5799-5806. |
入库方式: OAI收割
来源:上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。