Characterization of salvicine-resistant lung adenocarcinoma A549/SAL cell line
文献类型：期刊论文
| 作者 | Miao, ZH; Tong, LJ; Zhang, JS; Han, JX; Ding, J
|
| 刊名 | INTERNATIONAL JOURNAL OF CANCER
 |
| 出版日期 | 2004-07-10 |
| 卷号 | 110期号:5页码:627-632 |
| 关键词 | salvicine A549/SAL cell line antineoplastic agents drug resistance |
| ISSN号 | 0020-7136 |
| DOI | 10.1002/jc.20026 |
| 文献子类 | Article |
| 英文摘要 | Salvicine is a diterpenoid quinone derived from a traditional Chinese medication that has been shown to possess potent in vitro and in vivo antitumor effects. This compound, which inhibits the activity of Topoisomerase II, was found to equipotently kill various multidrug-resistant tumor cells and their corresponding parental counterparts in vitro and to inhibit mdrl/P-gp expression in multidrug-resistant K562/ A02 cells. To examine the features of tumor resistance to salvicine, we established a salvicine-resistant tumor cell subline of A549 lung adenocarcinoma cells. Compared with parental cells, A549/SAL cells displayed 8.91-fold resistance to salvicine and an average of 6.70-fold resistance to the anti-metabolites. A549/SAL cells, however, were not resistant to alkylating agents, platinum compounds and other naturally-derived antineoplastics. RT-PCR analysis showed that the expression of mRNAs from the mdr-1, MRP, PCNA, topoisomerase II alpha and beta, GSTpi, p21 and GADD45 genes was not altered in the salvicine-resistant subline. In contrast, expression of p53 and box mRNA was significantly lower, and expression of mdm2 mRNA was significantly higher, in A549/ SAL cells compared to A549 cells. A549/SAL cells grew more slowly, and in a more scattered pattern, than A549 cells. In addition, the A549/SAL cells showed enhanced ability to migrate and invade in comparison to the parental cells. These results indicate that exposure to salvicine does not induce a tumor multidrug-resistant phenotype. (C) 2004 Wiley-Liss, Inc. |
| WOS关键词 | GENE-EXPRESSION ; MULTIDRUG-RESISTANCE ; CARCINOMA-CELLS ; IN-VITRO ; CANCER ; APOPTOSIS ; ACTIVATION ; CYTOTOXICITY ; INDUCTION ; LEUKEMIA |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000221984900001 |
| 出版者 | WILEY-LISS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274047]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, J |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Div Antitumor Pharmacol, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Dept Phytochem, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, ZH,Tong, LJ,Zhang, JS,et al. Characterization of salvicine-resistant lung adenocarcinoma A549/SAL cell line[J]. INTERNATIONAL JOURNAL OF CANCER,2004,110(5):627-632. |
| APA | Miao, ZH,Tong, LJ,Zhang, JS,Han, JX,&Ding, J.(2004).Characterization of salvicine-resistant lung adenocarcinoma A549/SAL cell line.INTERNATIONAL JOURNAL OF CANCER,110(5),627-632. |
| MLA | Miao, ZH,et al."Characterization of salvicine-resistant lung adenocarcinoma A549/SAL cell line".INTERNATIONAL JOURNAL OF CANCER 110.5(2004):627-632. |
入库方式： OAI收割
来源：上海药物研究所
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