Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch
文献类型：期刊论文
| 作者 | Li, MH; Zhang, NX ; Chen, XQ; Wu, G; Wu, HM; Hu, GY
|
| 刊名 | TOXICON
 |
| 出版日期 | 2004-06-15 |
| 卷号 | 43期号:8页码:895-900 |
| 关键词 | BmKK2 Buthus martensi Karsch delayed rectifier K+ current hippocampus neurotoxin scorpion |
| ISSN号 | 0041-0101 |
| DOI | 10.1016/j.toxicon.2003.11.028 |
| 文献子类 | Article |
| 英文摘要 | BmKK2 (alpha-KTx 14.2) is one of the novel short-chain peptides found in molecular cloning of a venom gland cDNA library from Asian scorpion Buthus martensi Karsch. Based upon its amino acid sequence, the peptide was proposed to adopt a classical alpha/beta-scaffold for alpha-KTxs. In the present study, we purified BmKK2 from the venom of B. martensi Karsch, and investigated its action on voltage-dependent K+ currents in dissociated hippocampal neurons from neonatal rats. BmKK2 (10-100 muM) selectively inhibited the delayed rectifier K+ current, but did not affect the fast transient K+ current. The inhibition of BmKK2 on the delayed rectifier K+ current was reversible and voltage-independent. The peptide did not affect the steady-state activation of the current, but caused a depolarizing shift (about 9 mV) of its steady-state inactivation curve. The results demonstrate that BmKK2 is a novel K+ channel-blocking scorpion peptide. (C) 2004 Elsevier Ltd. All rights reserved. |
| WOS关键词 | SHAKER K+ CHANNEL ; TOXINS ; CHARYBDOTOXIN ; HIPPOCAMPAL ; RESIDUES ; SURFACE ; NEURONS ; PORE |
| WOS研究方向 | Pharmacology & Pharmacy ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000222564500005 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274061]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Wu, HM |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, MH,Zhang, NX,Chen, XQ,et al. Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch[J]. TOXICON,2004,43(8):895-900. |
| APA | Li, MH,Zhang, NX,Chen, XQ,Wu, G,Wu, HM,&Hu, GY.(2004).Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch.TOXICON,43(8),895-900. |
| MLA | Li, MH,et al."Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch".TOXICON 43.8(2004):895-900. |
入库方式： OAI收割
来源：上海药物研究所
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