Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones
文献类型：期刊论文
| 作者 | Zhang, T; Li, JL; Xin, J; Ma, XC; Tu, ZH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2004-02 |
| 卷号 | 25期号:2页码:171-175 |
| 关键词 | fluoroquinolones phototoxic dermatitis comet assay |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones (FQ). METHODS: Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and levofloxacin (LELX) were tested by comet assay and photodynamic DNA strand breaking activity under the different conditions of UVA irradiation. RESULTS: In comet assay, photogenotoxicity was evident at SPFX 1 mg/L, LFLX 5 mg/L, and CPFX 5 mg/L, and LELX 10 mg/L. In photodynamic DNA srand-breaking activity, SPFX and LFLX induced the conversion of the supercoiled form into the nicked relaxed form at 10-50 mumol/L, while CPFX at 25 mumol/L and LELX at 50 mumol/L. CONCLUSION: There were good correlations between the two methods to detect DNA damage induced by phototoxicity of fluoroquinolones. Photodynamic DNA strand breaking activity was a good method to detect DNA damage induced by photogenotoxicity of fluoroquinolones as well as comet assay. |
| WOS关键词 | IN-VITRO ; COMET ASSAY ; PHOTOTOXICITY ; ANTIBACTERIAL ; TESTS ; CELLS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 中文 |
| WOS记录号 | WOS:000188877300007 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274115]  |
| 专题 | 药物安全性评价中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第三研究室 |
| 通讯作者 | Tu, ZH |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, T,Li, JL,Xin, J,et al. Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones[J]. ACTA PHARMACOLOGICA SINICA,2004,25(2):171-175. |
| APA | Zhang, T,Li, JL,Xin, J,Ma, XC,&Tu, ZH.(2004).Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones.ACTA PHARMACOLOGICA SINICA,25(2),171-175. |
| MLA | Zhang, T,et al."Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones".ACTA PHARMACOLOGICA SINICA 25.2(2004):171-175. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。