中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus

文献类型:期刊论文

作者Sun, HF; Luo, HB; Yu, CY; Sun, T; Chen, J; Peng, SY; Qin, J; Shen, JH; Yang, YM; Xie, YH
刊名PROTEIN EXPRESSION AND PURIFICATION
出版日期2003-12
卷号32期号:2页码:302-308
关键词Severe Acute Respiratory Syndrome (SARS) SARS coronavirus 3C like protease molecular cloning expression purification mass spectrometric characterization
ISSN号1046-5928
DOI10.1016/j.pep.2003.08.016
文献子类Article
英文摘要Severe acute respiratory syndrome (SARS) is an acute respiratory illness, which has broken out in China. It has been known that SARS coronavirus (SARS_CoV) is a novel human coronavirus and is responsible for SARS infection. Belonging to one of the major proteins associated with SARS_CoV, SARS 3C-like protease (SARS_3CL(pro)) functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents. To facilitate the studies regarding the functions and structures of SARS_3CL(pro), in this report the synthetic genes encoding 3CL(pro), of SARS_CoV were assembled, and the plasmid was constructed using pQE30 as vector and expressed in Escherichia coli M15 cells. The highly yielded (similar to15 mg/L) expressed protease was purified by use of NTA-Ni2+ affinity chromatography and FPLC system, and its sequence was determined by LC/MS with the residue coverage of 46.4%. (C) 2003 Elsevier Inc. All rights reserved.
WOS关键词ACUTE RESPIRATORY SYNDROME ; VIRUS-ENCODED PROTEINASES ; RNA-POLYMERASE ; SEQUENCE ; IDENTIFICATION ; POLYPROTEIN ; KILOBASES ; DESIGN ; GENE-1 ; DOMAIN
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000187393600017
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/274155]  
专题新药研究国家重点实验室
中科院受体结构与功能重点实验室
通讯作者Jiang, HL
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Drug Discovery & Design Ct, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Sun, HF,Luo, HB,Yu, CY,et al. Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus[J]. PROTEIN EXPRESSION AND PURIFICATION,2003,32(2):302-308.
APA Sun, HF.,Luo, HB.,Yu, CY.,Sun, T.,Chen, J.,...&Jiang, HL.(2003).Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus.PROTEIN EXPRESSION AND PURIFICATION,32(2),302-308.
MLA Sun, HF,et al."Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus".PROTEIN EXPRESSION AND PURIFICATION 32.2(2003):302-308.

入库方式: OAI收割

来源:上海药物研究所

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