Apoptosis induced by beta-amyloid(25-35) in acetylcholinesterase-overexpressing neuroblastoma cells
文献类型：期刊论文
| 作者 | Zhang, HY ; Brimijoin, S; Tang, XC
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2003-09 |
| 卷号 | 24期号:9页码:853-858 |
| 关键词 | amyloid beta protein apoptosis acetylcholinesterase SC42 cells SC35 cells |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To examine the relationship between apoptosis induced by beta-amyloid fragment 25-35 (Abeta(25-35)) and the activity of acetylcholinesterase (AChE) in AChE over-expresser-SC42 cells. METHODS: Cell survival was measured by microscopy and MTT reduction; DNA laddering was observed by electrophoresis; AChE activity was determined by spectrophotometry. RESULTS: Abeta(25-35) I mumol/L exposure for 24-48 h caused a significant decrease in cell viability, along with changes in morphology and DNA fragmentation. AChE activity was affected in an inverse manner, increasing gradually to a level that was 1.7-fold higher than control at the 48-h time point. No change in the cytotoxicity of Abeta(25-35) was observed when the increased AChE activities were effectively inhibited by huperzine A throughout the 48-h exposure period. CONCLUSION: Although Abeta(25-35) can induce apoptosis in SC42 cells and simultaneously increase AChE activity, the capacity of AChE to hydrolyze acetylcholine is not involved in this apoptosis model. |
| WOS关键词 | ALZHEIMERS-DISEASE ; PROTEIN ; FIBRILS ; CHOLINESTERASES ; COLOCALIZATION ; PEPTIDES ; ENZYME ; SITE |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1270830 |
| WOS记录号 | WOS:000185357500003 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274187]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Brimijoin, S |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Mayo Clin, Dept Mol Pharmacol, Rochester, MN 55905 USA |
| 推荐引用方式 GB/T 7714 | Zhang, HY,Brimijoin, S,Tang, XC. Apoptosis induced by beta-amyloid(25-35) in acetylcholinesterase-overexpressing neuroblastoma cells[J]. ACTA PHARMACOLOGICA SINICA,2003,24(9):853-858. |
| APA | Zhang, HY,Brimijoin, S,&Tang, XC.(2003).Apoptosis induced by beta-amyloid(25-35) in acetylcholinesterase-overexpressing neuroblastoma cells.ACTA PHARMACOLOGICA SINICA,24(9),853-858. |
| MLA | Zhang, HY,et al."Apoptosis induced by beta-amyloid(25-35) in acetylcholinesterase-overexpressing neuroblastoma cells".ACTA PHARMACOLOGICA SINICA 24.9(2003):853-858. |
入库方式： OAI收割
来源：上海药物研究所
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