Effects of huperzine A on liver cytochrome P-450 in rats
文献类型：期刊论文
| 作者 | Ma, XC; Wang, HX; Xin, J; Zhang, T; Tu, ZH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2003-08 |
| 卷号 | 24期号:8页码:831-835 |
| 关键词 | huperzine A cytochrome P-450 cytochrome P-450CYP1A2 liver rat |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To predict possible drug interaction and assure safety medication of huperzine A (HupA). METHODS: The effects of HupA on activities and expressions of cytochrome P-450 (CYP) were examined. Liver microsomes and total mRNA were prepared from rats treated orally with 0, 0.1 (pharmacological dose), 1, or 2 mg/kg huperzine A for 2 weeks. Phenobarbital, 3-methylcholanthrene (3-MC), ethanol, and dexamethasone were used as positive controls. Total CYP protein was assayed by carbon monoxide difference spectrum. Activity of isoenzyme was detected with specific probe. Expression of CYP protein and mRNA was analyzed with Western blot and RT-PCR. RESULTS: No changes of isoenzyme expression and catalytic activities were found in rats treated with 0.1 mg/kg huperzine A. Huperzine A 1, 2 mg/kg parallelly increased CYP1A2 activity, protein and mRNA, although they were minor when contrasted with 3-MC. Huperzine A 1, 2 mg/kg got no effects on CYP2C11, CYP2B1/2, 2E1 and 3A. CONCLUSION: Activity and expression of liver CYP isoenzymes were not affected in rats treated with pharmacological dose of HupA, but HupA at toxicological dose may elicit a slight inductive response of CYP1A2. The CYP1A2 induction produced by HupA is related to transcription enhancement. |
| WOS关键词 | ALZHEIMERS-DISEASE ; OXIDATION ; ENZYMES ; MICROSOMES ; CHEMICALS ; TACRINE |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000184722600016 |
| 出版者 | SHANGHAI INST MATERIA MEDICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274199]  |
| 专题 | 药物安全性评价中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Tu, ZH |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Fudan Univ, Dept Pharmacol, Sch Pharm, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, XC,Wang, HX,Xin, J,et al. Effects of huperzine A on liver cytochrome P-450 in rats[J]. ACTA PHARMACOLOGICA SINICA,2003,24(8):831-835. |
| APA | Ma, XC,Wang, HX,Xin, J,Zhang, T,&Tu, ZH.(2003).Effects of huperzine A on liver cytochrome P-450 in rats.ACTA PHARMACOLOGICA SINICA,24(8),831-835. |
| MLA | Ma, XC,et al."Effects of huperzine A on liver cytochrome P-450 in rats".ACTA PHARMACOLOGICA SINICA 24.8(2003):831-835. |
入库方式： OAI收割
来源：上海药物研究所
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