Studies of marine sulfated polymannuroguluronate on endothelial cell proliferation and endothelial immunity and related mechanisms
文献类型：期刊论文
| 作者 | Wang, LC; Geng, MY ; Li, J; Guan, HS; Ding, J
|
| 刊名 | JOURNAL OF PHARMACOLOGICAL SCIENCES
 |
| 出版日期 | 2003-08 |
| 卷号 | 92期号:4页码:367-373 |
| 关键词 | sulfated polymannuroguluronate basic fibroblast growth factor intercellular adhesion molecule-1 endothelial cell angiogenesis |
| ISSN号 | 1347-8613 |
| 文献子类 | Article |
| 英文摘要 | Anti-proliferation action and enhancement of endothelial cell immunity and related mechanisms by marine sulfated polymannuroguluronate (SPMG) were investigated in the present studies. Endothelial cell proliferation was evaluated by MTT assay. Intercellular adhesion molecule-1 (ICAM-1) expression was analyzed by flow cytometry. The interaction of SPMG with basic fibroblast growth factor (bFGF) was evaluated by surface plasmon resonance. Results showed that SPMG exhibited a significant inhibitory effect against proliferation in both normal human umbilical vein endothelial cells (HUVEC) and bFGF-treated HUVEC, the action of which was completely abrogated by bFGF antibody. SPMG exerted high affinity to bFGF in a multivalent pattern, characterized by one molecule SPMG binding to 3 - 4 molecules of bFGF. Moreover, SPMG enhanced ICAM-1 expression in HUVEC and prevented and restored bFGF-treated downregulation of ICAM-1 expression in HUVEC, the expression of which was not counteracted by bFGF antibody. In conclusion, this is the first report demonstrating that SPMG exerted an anti-proliferation effect dependent on the bFGF-regulated pathway and afforded upregulatory activity on ICAM-1 expression regardless of the involvement of bFGF. |
| WOS关键词 | FIBROBLAST-GROWTH-FACTOR ; ANGIOGENESIS FACTORS ; HEPARIN ; BINDING ; BFGF ; EXPRESSION ; RECEPTOR ; INHIBITORS ; ACTIVATION ; MOLECULES |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000185033600008 |
| 出版者 | JAPANESE PHARMACOLOGICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274202]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Geng, MY |
| 作者单位 | 1.Ocean Univ China, Marine Drug & Food Inst, Dept Marine Pharmacol, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Materia Med, State Key Lab Drug Res,Div Antitumour Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, LC,Geng, MY,Li, J,et al. Studies of marine sulfated polymannuroguluronate on endothelial cell proliferation and endothelial immunity and related mechanisms[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2003,92(4):367-373. |
| APA | Wang, LC,Geng, MY,Li, J,Guan, HS,&Ding, J.(2003).Studies of marine sulfated polymannuroguluronate on endothelial cell proliferation and endothelial immunity and related mechanisms.JOURNAL OF PHARMACOLOGICAL SCIENCES,92(4),367-373. |
| MLA | Wang, LC,et al."Studies of marine sulfated polymannuroguluronate on endothelial cell proliferation and endothelial immunity and related mechanisms".JOURNAL OF PHARMACOLOGICAL SCIENCES 92.4(2003):367-373. |
入库方式： OAI收割
来源：上海药物研究所
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