Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity
文献类型：期刊论文
| 作者 | Qing, C; Miao, ZH; Tong, LJ; Zhang, JS; Ding, J
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2003-05 |
| 卷号 | 24期号:5页码:415-421 |
| ISSN号 | 1671-4083 |
| 关键词 | salvicine actinomycin D K562 cells cytotoxicity apoptosis |
| 文献子类 | Article |
| 英文摘要 | Aim: To study the effects of actinomycin D (Act D) on the cytotoxicity and apoptosis elicited by salvicine in human leukemia K562 cells. Methods: Growth inhibition of K562 cells was measured by the microculture tetrozolium (MTT) assay. Cell apoptosis was evaluated by fluorescence microscopy, DNA agarose gel electrophoresis, and flow cytometry. Results: Following exposure of K-562 cells to salvicine plus Act D for 24 h, Act D at the concentrations of 0.04, 0.4, and 4 mumol/L potentiated the cytotoxicity of salvicine 6.25 mumol/L to, some degree. The mean growth inhibitory rates went from 8% up to 69%, 71%, and 70%, respectively. However, the same enhancement of Act D did not continue to emerge at the higher concentrations than salvicine 6.25 mumol/L. Act D enhanced, or at least, did not decrease the cytotoxicity of salvicine against K562 cells. Fluorescence microscopy, DNA agarose gel electrophoresis, and flow cytometry revealed that Act D concentration-dependently inhibited the induction of apoptosis by salvicine in the same cell line. Conclusion: The combination of salvicine and Act D in a proper range of concentrations is able to enhance the cytotoxicity of salvicine against K562 cells though inhibiting apoptosis. The other mechanisms of cell death except apoptosis may be implicated in the process. |
| WOS关键词 | MEDIATED APOPTOSIS ; CANCER CELLS ; DNA ; INDUCTION ; EXPRESSION ; CARCINOMA ; SURVIVAL ; MYELOMA ; AGENTS ; ASSAY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:510065 |
| 出版者 | SHANGHAI INST MATERIA MEDICA |
| WOS记录号 | WOS:000182553500007 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274236]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, J |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Div Antitumor Ppharmacol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Dept Phytochem, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qing, C,Miao, ZH,Tong, LJ,et al. Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity[J]. ACTA PHARMACOLOGICA SINICA,2003,24(5):415-421. |
| APA | Qing, C,Miao, ZH,Tong, LJ,Zhang, JS,&Ding, J.(2003).Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity.ACTA PHARMACOLOGICA SINICA,24(5),415-421. |
| MLA | Qing, C,et al."Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity".ACTA PHARMACOLOGICA SINICA 24.5(2003):415-421. |
入库方式： OAI收割
来源：上海药物研究所
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