Tacrine attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells
文献类型：期刊论文
| 作者 | Wang, R; Zhou, J; Tang, XC
|
| 刊名 | MOLECULAR BRAIN RESEARCH
 |
| 出版日期 | 2002-10-30 |
| 卷号 | 107期号:1页码:1-8 |
| 关键词 | tacrine oxidative stress apoptosis apoptosis-related genes PC12 cells |
| ISSN号 | 0169-328X |
| DOI | 10.1016/S0169-328X(02)00402-3 |
| 文献子类 | Article |
| 英文摘要 | The present studies investigated the effects of tacrine, a selective acetylcholinesterase (AChE) inhibitor and promising anti-dementia agent, on hydrogen peroxide (H2O2)-induced apoptosis and the expression of apoptosis-related genes in rat pheochromocytoma line PC12 cells. Transient exposure of the cells to H2O2 (100 muM) triggered typical apoptosis as evidenced by chromatin condensation, nuclei fragmentation and DNA laddering. RT-PCR studies showed upregulated p53 and bax mRNA levels with H2O2 treatment. The results were further confirmed at protein levels by immunocytochemistry with specific antibodies. Preincubation with tacrine significantly attenuated H2O2-induced injury, prevented the cells from apoptosis and attenuated H2O2-induced overexpression of bax and p53. The present findings suggest that tacrine exert significant protection against H2O2-induced apoptosis possibly through inhibiting expression of pro-apoptosis genes. (C) 2002 Elsevier Science B.V. All rights reserved. |
| WOS关键词 | MITOCHONDRIAL PERMEABILITY TRANSITION ; AMYLOID PRECURSOR PROTEIN ; ALZHEIMERS-DISEASE ; OXIDATIVE STRESS ; DNA FRAGMENTATION ; TETRAHYDROAMINOACRIDINE ; ACETYLCHOLINESTERASE ; DEATH ; P53 ; BUTYRYLCHOLINESTERASE |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000179450500001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274316]  |
| 专题 | 院士及顾问专家 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Tang, XC |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, R,Zhou, J,Tang, XC. Tacrine attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells[J]. MOLECULAR BRAIN RESEARCH,2002,107(1):1-8. |
| APA | Wang, R,Zhou, J,&Tang, XC.(2002).Tacrine attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells.MOLECULAR BRAIN RESEARCH,107(1),1-8. |
| MLA | Wang, R,et al."Tacrine attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells".MOLECULAR BRAIN RESEARCH 107.1(2002):1-8. |
入库方式： OAI收割
来源：上海药物研究所
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