Mapping the binding site of p53 on UBC9 by NMR spectroscopy
文献类型：期刊论文
| 作者 | Lin, DH |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2002-10 |
| 卷号 | 20期号:10页码:937-943 |
| 关键词 | NMR protein-peptide interaction SUMO-1 pathway E2 enzyme p53 |
| ISSN号 | 1001-604X |
| 文献子类 | Article |
| 英文摘要 | Human UBC9 is a member of the E2 family of proteins. However, Instead of conjugating to ubiquitin, it conjugates to a ubiquitin homologue SUMO-1 (also known as UBL1, GMP1, SMTP3, PICT-1 and sentrin). The SUMO-1 conjugation pathway is very similar to that of ubiquitin with regard to the primary sequences of the ubiquitin activating enzymes (E1), the three-dimensional structures of the ubiquitin conjugating enzymes (E2), and the chemistry of the overall conjugation pathway. The interaction of p53 and UBC9, the E2 of the SUMO-1 pathway, has been studied by unclear magnetic resonance spectroscopy. A peptide corresponding to the nuclear localization domain of p53 specifically interacts with UBC9 and this interaction is likely to be important for conjugation of p53 with SUMO-1. The largest chemical shift changes on UBC9 occur at residues 94 and 129-135. This region is adjacent to the active site and has significant dynamic behavior on the mus-ms and ps-ns timescales. Correlation of chemical shift changes and mobility of these residues further suggest the importance of these residues in substrate recognition. |
| WOS关键词 | UBIQUITIN-CONJUGATING ENZYME ; CRYSTAL-STRUCTURE ; SACCHAROMYCES-CEREVISIAE ; ANGSTROM RESOLUTION ; PROTEINS ; SUMO-1 ; SYSTEM |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000178644700003 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274323]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Lin, DH |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Meteria Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, DH. Mapping the binding site of p53 on UBC9 by NMR spectroscopy[J]. CHINESE JOURNAL OF CHEMISTRY,2002,20(10):937-943. |
| APA | Lin, DH.(2002).Mapping the binding site of p53 on UBC9 by NMR spectroscopy.CHINESE JOURNAL OF CHEMISTRY,20(10),937-943. |
| MLA | Lin, DH."Mapping the binding site of p53 on UBC9 by NMR spectroscopy".CHINESE JOURNAL OF CHEMISTRY 20.10(2002):937-943. |
入库方式： OAI收割
来源：上海药物研究所
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