Clean SEA-HSQC: A method to map solvent exposed amides in large non-deuterated proteins with gradient-enhanced HSQC
文献类型：期刊论文
| 作者 | Lin, DH; Sze, KH; Cui, YF; Zhu, G |
| 刊名 | JOURNAL OF BIOMOLECULAR NMR
 |
| 出版日期 | 2002-08 |
| 卷号 | 23期号:4页码:317-322 |
| 关键词 | amide hydrogen exchange CLEAN-PM HSQC proteins resonance overlap solvent exposed amides |
| ISSN号 | 0925-2738 |
| DOI | 10.1023/A:1020225206644 |
| 文献子类 | Article |
| 英文摘要 | The recent introduction of the SEA-TROSY experiment (Pellecchia et al. (2001) J. Am. Chem. Soc., 123, 4633-4634) can alleviate the problem of resonance overlap in N-15/H-2 labeled proteins. This method selectively observes solvent exposed amide protons with a SEA element. However, SEA-TROSY spectra may be contaminated with exchange-relayed NOE contributions from fast exchanging hydroxyl or amine protons and longitudinal relaxation contributions. Furthermore, for non-deuterated proteins or protein-ligand complexes, SEA-TROSY spectra may contain NOE contributions from aliphatic protons. In this communication, a modified version of the SEA element, a Clean SEA element, is introduced to eliminate these artifacts. |
| WOS关键词 | PROTON-EXCHANGE RATES ; HYDROGEN-EXCHANGE ; NMR-SPECTROSCOPY ; WATER ; FILTERS ; SCHEME ; TROSY ; NOES ; PM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000177984600007 |
| 出版者 | KLUWER ACADEMIC PUBL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274342]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Zhu, G |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Meteria Med, State Key Lab Drug Res,Ctr Drug Discovery & Desig, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, DH,Sze, KH,Cui, YF,et al. Clean SEA-HSQC: A method to map solvent exposed amides in large non-deuterated proteins with gradient-enhanced HSQC[J]. JOURNAL OF BIOMOLECULAR NMR,2002,23(4):317-322. |
| APA | Lin, DH,Sze, KH,Cui, YF,&Zhu, G.(2002).Clean SEA-HSQC: A method to map solvent exposed amides in large non-deuterated proteins with gradient-enhanced HSQC.JOURNAL OF BIOMOLECULAR NMR,23(4),317-322. |
| MLA | Lin, DH,et al."Clean SEA-HSQC: A method to map solvent exposed amides in large non-deuterated proteins with gradient-enhanced HSQC".JOURNAL OF BIOMOLECULAR NMR 23.4(2002):317-322. |
入库方式： OAI收割
来源：上海药物研究所
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