Identification of three sulfate-conjugated metabolites of berberine chloride in healthy volunteers' urine after oral administration
文献类型：期刊论文
| 作者 | Pan, JF; Yu, C; Zhu, DY; Zhang, H; Zeng, JF; Jiang, SH; Ren, JY |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2002-01 |
| 卷号 | 23期号:1页码:77-82 |
| 关键词 | berberine biotransformation metabolism biomolecular nuclear magnetic resonance electrospray ionization mass spectrometry |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To identify the structure of unknown metabolites of berberine (Ber) in human urine after oral administration. METHODS : Urine samples were obtained from 5 volunteers after they orally took Ber chloride 0.9 g per day for three days. Metabolites in urine samples were isolated and purified by polyporous resin column chromatography. The individual metabolites were identified mainly using electrospray ionization mass spectroscopy (ESI-MS) and proton nuclear magnetic resonance (H-1 NMR) spectroscopy. RESULTS: Three unknown metabolites (M1, M2, and M3) were isolated. They were susceptible to arylsufatase. ESI-MS measurements of M1, M2, and M3 produced quasimolecular ions [M + H](+), m/z 417.9, 404.0, and 402.0 respectively. Especially, each of them produced a characteristic protonated ion [M-80 + H](+), which can be ascribed as quasimolecular ions lost a SO3 fragment. H-1 NMR spectra of the metabolites were also obtained and each of H-1 signals was assigned. CONCLUSION: Structures of M1, M2, and M3 were firmly identified as jatrorrhizine-3-sulfate, demethyleneberberine-2-sulfate, and thalifendine-10-sulfate, and the major metabolite was M2. |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1366764 |
| WOS记录号 | WOS:000173350900013 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274406]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Zhu, DY |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Shanghai Xu Hui Dist Cent Hosp, Res Ctr, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, JF,Yu, C,Zhu, DY,et al. Identification of three sulfate-conjugated metabolites of berberine chloride in healthy volunteers' urine after oral administration[J]. ACTA PHARMACOLOGICA SINICA,2002,23(1):77-82. |
| APA | Pan, JF.,Yu, C.,Zhu, DY.,Zhang, H.,Zeng, JF.,...&Ren, JY.(2002).Identification of three sulfate-conjugated metabolites of berberine chloride in healthy volunteers' urine after oral administration.ACTA PHARMACOLOGICA SINICA,23(1),77-82. |
| MLA | Pan, JF,et al."Identification of three sulfate-conjugated metabolites of berberine chloride in healthy volunteers' urine after oral administration".ACTA PHARMACOLOGICA SINICA 23.1(2002):77-82. |
入库方式： OAI收割
来源：上海药物研究所
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