Effect of epristeride on the expression of IGF-1 and TGF-beta receptors in androgen-induced castrated rat prostate
文献类型：期刊论文
| 作者 | Wu, SF; Sun, HZ; Qi, XD; Tu, ZH |
| 刊名 | EXPERIMENTAL BIOLOGY AND MEDICINE
 |
| 出版日期 | 2001-11 |
| 卷号 | 226期号:10页码:954-960 |
| 关键词 | epristeride rat prostate growth factor immunohistochemistry in situ hybridization |
| ISSN号 | 0037-9727 |
| 文献子类 | Article |
| 英文摘要 | The development of benign prostatic hyperplasia (BPH) is an androgen-dependent process that may be mediated by a number of locally produced growth factors. Among them, insulinlike growth factor 1 (IGF-1) and transforming growth factor beta (TGF beta) are thought important in regulating prostate growth and homeostasis, and their expression undergoes changes in proliferative prostatic disease. Epristeride, a 5 alpha -reductase inhibitor, is an, effective drug in the treatment of BPH, inducing regressive changes in the prostate. This study was designed to assess the effects, of epristeride on expression of these two factors at mRNA and protein levels in castrated rats maintained with exogenous testosterone. Epristeride treatment caused significant reduction in ventral prostate weight in a dose-dependent manner. There was a positive correlation between IGF-1 mRNA expression and ventral prostate weight and an inverse, correlation between TGF-beta1 mRNA expression and ventral prostate. weight. Immunohistochemistry showed strong IGF-1 receptor immunoreactivity in the prostatic epithelial cells of untreated animals. In situ hybridization demonstrated high levels, of IGF-1 mRNA expression both in the prostatic stromal and epithelial cells, of untreated rats. In treated rats, both IGF-1 receptor protein and; IGF-1 mRNA levels decreased significantly, and IGF-1 mRNA was mainly expressed in prostatic stromal cells. Weak expression; of TGF beta receptors at the protein level and TWO at the mRNA level were found in the prostatic hyperplastic epithelial cells of untreated rats. In treated animals, intense T beta RII immunoreactivity was, observed in epithelial cells, and a higher level of TGF beta mRNA was observed in both epithelial cells and stromal cells compared with control animals. In our opinion, the effect of epristeride on rat prostatic, atrophy might be mediated via local growth factor(s). |
| WOS关键词 | GROWTH-FACTOR-BETA ; PROGRAMMED CELL-DEATH ; EPITHELIAL-CELLS ; VENTRAL PROSTATE ; FACTOR-I ; BINDING-PROTEIN ; HYPERPLASIA ; INSULIN ; LOCALIZATION ; TISSUE |
| WOS研究方向 | Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000171889700012 |
| 出版者 | SOC EXPERIMENTAL BIOLOGY MEDICINE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274437]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Tu, ZH |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Fudan Univ, Med Ctr, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, SF,Sun, HZ,Qi, XD,et al. Effect of epristeride on the expression of IGF-1 and TGF-beta receptors in androgen-induced castrated rat prostate[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2001,226(10):954-960. |
| APA | Wu, SF,Sun, HZ,Qi, XD,&Tu, ZH.(2001).Effect of epristeride on the expression of IGF-1 and TGF-beta receptors in androgen-induced castrated rat prostate.EXPERIMENTAL BIOLOGY AND MEDICINE,226(10),954-960. |
| MLA | Wu, SF,et al."Effect of epristeride on the expression of IGF-1 and TGF-beta receptors in androgen-induced castrated rat prostate".EXPERIMENTAL BIOLOGY AND MEDICINE 226.10(2001):954-960. |
入库方式： OAI收割
来源：上海药物研究所
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