Knockdown of IGF- I R by antisense oligodeoxynucleotide auguments sensitivity of T24 bladder cancer cells to mitomycin
文献类型：期刊论文
| 作者 | Sun, HZ; Wu, SF; Tu, ZH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001-09 |
| 卷号 | 22期号:9页码:841-846 |
| 关键词 | insulin-like growth factor I receptors autocrine drug resistance apoptosis bladder neoplasms |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To investigate whether insulin-like growth factor receptor (IGF-I R) was involved in drug, resistance of bladder cancer cells. METHODS: RT-PCR was used to detect the mRNA expression of IGF-I, IGF-II, and IGF-I R in T24 cells and normal urothelial cells. Flow cytometry and NM tests were used to assess the effect of antisense oligodeoxynucleotide ( ODN) on drug sensitivities and apoptosis of T24 cells to mitomycin (NMC). Western blot was used to analyze the effect of ODN on expression of IGF-I R protein. RESULTS: mRNA of IGF-I, IGF-II, and IGF-I R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace levels; knockdown of IGF-I R by antisense ODN greatly inhibited the growth of bladder cancer cells and enhanced sensitivity and apoptosis of T24 cells to MMC. CONCLUSION: These results suggested that blockage of IGF-I R signaling might potentially contribute to the treatment of bladder cancer cells which were insensitive to chemotherapy. |
| WOS关键词 | RESISTANCE-ASSOCIATED PROTEIN ; GROWTH-FACTOR ; INDUCED APOPTOSIS ; TUMOR-CELLS ; RECEPTOR ; INHIBITION ; MODULATION ; EXPRESSION |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000171146200014 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274450]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Tu, ZH |
| 作者单位 | 1.Weizmann Inst Sci, Dept Regulat Biol, IL-76100 Rehovot, Israel 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, HZ,Wu, SF,Tu, ZH. Knockdown of IGF- I R by antisense oligodeoxynucleotide auguments sensitivity of T24 bladder cancer cells to mitomycin[J]. ACTA PHARMACOLOGICA SINICA,2001,22(9):841-846. |
| APA | Sun, HZ,Wu, SF,&Tu, ZH.(2001).Knockdown of IGF- I R by antisense oligodeoxynucleotide auguments sensitivity of T24 bladder cancer cells to mitomycin.ACTA PHARMACOLOGICA SINICA,22(9),841-846. |
| MLA | Sun, HZ,et al."Knockdown of IGF- I R by antisense oligodeoxynucleotide auguments sensitivity of T24 bladder cancer cells to mitomycin".ACTA PHARMACOLOGICA SINICA 22.9(2001):841-846. |
入库方式： OAI收割
来源：上海药物研究所
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