Huperzine A attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells
文献类型：期刊论文
| 作者 | Wang, R; Xiao, XQ; Tang, XC
|
| 刊名 | NEUROREPORT
 |
| 出版日期 | 2001-08-28 |
| 卷号 | 12期号:12页码:2629-2634 |
| 关键词 | apoptosis apoptosis-related genes huperzine A oxidative stress PC12 cells |
| ISSN号 | 0959-4965 |
| DOI | 10.1097/00001756-200108280-00009 |
| 文献子类 | Article |
| 英文摘要 | The present studies investigated effects of huperzine A (HupA), a selective acetylcholinesterase (AChE) inhibitor and promising anti-dementia agent, On hydrogen peroxide (H(2)O(2))-induced apoptosis and the expression of apoptosis-related genes in rat pheochromocytoma line PC12 cells. Transient exposure of the cells to H(2)O(2) (100 muM) triggered a typical apoptosis as evidenced by chromatin condensation, nuclei fragmentation and DNA laddering. RT-PCR studies showed up-regulated p53 and Bax but lowered Bcl-2 mRNA levels with H(2)O(2) treatment. The results were further confirmed at protein levels by immunocytochemistry with specific antibodies. Preincubation with HupA (1 muM) significantly prevented the cells from apoptosis, attenuated H(2)O(2)-induced over-expression of Bax and p53, and rehabilitated the level of Bcl-2. The present findings suggest that HupA exerts significant protection against H(2)O(2)-induced apoptosis, possibly through improving expression of apoptosis-related genes. NeuroReport 12:2629-2634 (C) 2001 Lippincott Williams & Wilkins. |
| WOS关键词 | MITOCHONDRIAL PERMEABILITY TRANSITION ; ALZHEIMERS-DISEASE ; OXIDATIVE STRESS ; DNA FRAGMENTATION ; DEATH ; TACRINE ; PROTEINS ; BCL-2 ; BAX |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000170600500009 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274456]  |
| 专题 | 院士及顾问专家 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Tang, XC |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, R,Xiao, XQ,Tang, XC. Huperzine A attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells[J]. NEUROREPORT,2001,12(12):2629-2634. |
| APA | Wang, R,Xiao, XQ,&Tang, XC.(2001).Huperzine A attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells.NEUROREPORT,12(12),2629-2634. |
| MLA | Wang, R,et al."Huperzine A attenuates hydrogen peroxide-induced apoptosis by regulating expression of apoptosis-related genes in rat PC12 cells".NEUROREPORT 12.12(2001):2629-2634. |
入库方式： OAI收割
来源：上海药物研究所
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