Antiangiogenic potential of 10-hydroxycamptothecin
文献类型：期刊论文
| 作者 | Xiao, D; Tan, WF; Li, MH; Ding, J
|
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2001-08-24 |
| 卷号 | 69期号:14页码:1619-1628 |
| 关键词 | 10-hydroxycamptothecin angiogenesis inhibitor human microvascular endothelial cells CAM assay apoptosis |
| ISSN号 | 0024-3205 |
| DOI | 10.1016/S0024-3205(01)01236-X |
| 文献子类 | Article |
| 英文摘要 | To investigate the antiangiogenic potential of 10-hydroxycamptothecin (HCPT), the proliferation of human microvascular endothelial cells (HMEC) and seven human tumor cell lines were detected by SRB assay, and the endothelial cell migration and tube formation were assessed using two in vitro model systems. Also, inhibition of angiogenesis was determined with a modification of the chick embryo chorioallantoic membrane (CAM) assay in vivo. Morphological assessment of apoptosis was performed by fluorescence microscope. HCPT 0.313-5 mu mol.L-1 treatment resulted in a dose-dependent inhibition of proliferation, migration and tube formation in HMEC cells, and HCPT 6.25-25 nmol.egg(-1) inhibited angiogenesis in CAM assay. HCPT 1.25-5 mu mo1.L-1 elicited typical morphological changes of apoptosis including condensed chromatin, nuclear fragmentation, and reduction in volume in HMEC cells. HCPT significantly inhibited angiogenesis both in vitro and in vivo at relatively low concentrations, and this effect was related with induction of apoptosis in HMEC cells. These results taken collectively suggest that HCPT may be a potent antiangiogenetic and cytotoxic drug and further investigation is warranted. (C) 2001 Elsevier Science Inc. All rights reserved. |
| WOS关键词 | HEP G2 CELLS ; ENDOTHELIAL-CELLS ; TOPOISOMERASE-I ; TUBE FORMATION ; APOPTOSIS ; ANGIOGENESIS ; CAMPTOTHECIN ; TOPOTECAN ; INHIBITOR ; DRUGS |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000170739300004 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274457]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, J |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, Shanghai Inst Biol Sci,State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, D,Tan, WF,Li, MH,et al. Antiangiogenic potential of 10-hydroxycamptothecin[J]. LIFE SCIENCES,2001,69(14):1619-1628. |
| APA | Xiao, D,Tan, WF,Li, MH,&Ding, J.(2001).Antiangiogenic potential of 10-hydroxycamptothecin.LIFE SCIENCES,69(14),1619-1628. |
| MLA | Xiao, D,et al."Antiangiogenic potential of 10-hydroxycamptothecin".LIFE SCIENCES 69.14(2001):1619-1628. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。