Effect of epristeride on expression of TGF-beta receptor in rat prostatic epithelial cells in vitro
文献类型：期刊论文
| 作者 | Wu, SF; Sun, HZ; Tu, ZH; Wu, HY |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001-06 |
| 卷号 | 22期号:6页码:516-520 |
| 关键词 | prostatic hyperplasia epristeride rats transforming growth factor beta receptors reverse transcriptase polymerase chain reaction Western blotting |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To assess the effect of epristeride on the expression of transforming growth factor P type ii receptor (TPR II) in rat prostatic epithelial cells in vitro. METHODS: RT-PCR and Western blot were used to quantitatively detect the mRNA and protein expressions of T betaR II in rat prostatic epithelial cells treated or untreated with epristeride. Immunocytochemical staining method was used to qualitatively analyze the expression of T betaR II protein. RESULTS: After treatment with epristeride 180 or 360 nmol/L, T betaR II mRNA expression levels were 0.56 +/- 0.08 and 0.59 +/- 0.07, respectively, which were significantly up-regulated compared with control cells (0.38 +/- 0.04, P < 0.05); expression level of TPR II protein were 3163 +/- 920 and 6769 +/- 1941, respectively, which were also markedly up-regulated compared with control cells (536 +/- 240, P < 0.05). Immunostaining showed weak positive reaction in control cells, while strong staining of TPR II was found in epristeride-treated cells. CONCLUSION: Epristeride may upregulate the expression of TPR II to induce apoptosis of prostatic epithelial cells. |
| WOS关键词 | GROWTH-FACTOR-BETA ; VENTRAL PROSTATE ; DEATH ; HYPERPLASIA ; CULTURE ; PROLIFERATION ; CASTRATION |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:515836 |
| WOS记录号 | WOS:000169174700007 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274484]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Tu, ZH |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Sci, Shanghai Inst Materia Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China 3.Fudan Univ, Shanghai Peoples Hosp 1, Med Ctr, Dept Urol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, SF,Sun, HZ,Tu, ZH,et al. Effect of epristeride on expression of TGF-beta receptor in rat prostatic epithelial cells in vitro[J]. ACTA PHARMACOLOGICA SINICA,2001,22(6):516-520. |
| APA | Wu, SF,Sun, HZ,Tu, ZH,&Wu, HY.(2001).Effect of epristeride on expression of TGF-beta receptor in rat prostatic epithelial cells in vitro.ACTA PHARMACOLOGICA SINICA,22(6),516-520. |
| MLA | Wu, SF,et al."Effect of epristeride on expression of TGF-beta receptor in rat prostatic epithelial cells in vitro".ACTA PHARMACOLOGICA SINICA 22.6(2001):516-520. |
入库方式： OAI收割
来源：上海药物研究所
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