Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats
文献类型：期刊论文
| 作者 | Leng, Y ; Gu, ZP; Cao, L
|
| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2000-12-08 |
| 卷号 | 409期号:2页码:123-131 |
| 关键词 | droloxifene luteal cell apoptosis c-myc bax bcl-2 mRNA |
| ISSN号 | 0014-2999 |
| DOI | 10.1016/S0014-2999(00)00826-8 |
| 文献子类 | Article |
| 英文摘要 | Droloxifene is a tamoxifen derivative whose effects in the therapy of human breast cancer and postmenopausal osteoporosis have been studied widely. We had found that droloxifene could induce apoptosis of luteal cells of rat in vitro, but its mechanisms were unknown. In the present study, the expression of c-myc, bax and bcl-2 mRNA in cultured rat luteal cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed. Cultured luteal cells of rats were incubated with droloxifene at various concentrations and with treatment durations. Occurrence of apoptosis was detected by terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL), DNA staining and DNA electrophoresis. Expression of these genes' mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the c-myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased, while the bcl-2 mRNA level exhibited no changes. A marked increase of c-myc and bax mRNA appeared respectively with 12 and 24 h of treatment, while a clear increase of apoptosis of luteal cells was found at 18 h. These results suggested that droloxifene could induce apoptosis of luteal cells of rat in vitro. The increase of c-myc mRNA expression might be one of the initiating factors and the elevated ratio of bax/bcl-2 mRNA was also probably involved in this effect. (C) 2000 Elsevier Science B.V. All rights reserved. |
| WOS关键词 | MESSENGER-RIBONUCLEIC-ACID ; RABBIT CORPUS-LUTEUM ; CORPORA-LUTEA ; HUMAN OVARY ; IN-VIVO ; FOLLICULAR-GROWTH ; GRANULOSA-CELLS ; DEATH ; REGRESSION ; LUTEOLYSIS |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000165774000004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274546]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Gu, ZP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Leng, Y,Gu, ZP,Cao, L. Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2000,409(2):123-131. |
| APA | Leng, Y,Gu, ZP,&Cao, L.(2000).Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats.EUROPEAN JOURNAL OF PHARMACOLOGY,409(2),123-131. |
| MLA | Leng, Y,et al."Apoptosis induced by droloxifene and c-myc, bax and bcl-2 mRNA expression in cultured luteal cells of rats".EUROPEAN JOURNAL OF PHARMACOLOGY 409.2(2000):123-131. |
入库方式： OAI收割
来源：上海药物研究所
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