Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch
文献类型：期刊论文
| 作者 | Wu, G; Li, YM; Wei, DS; He, FH; Jiang, SK; Hu, GY; Wu, HM |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2000-10-05 |
| 卷号 | 276期号:3页码:1148-1154 |
| 关键词 | scorpion toxin Buthus martensii Karsch NMR K+ channel blocker |
| ISSN号 | 0006-291X |
| DOI | 10.1006/bbrc.2000.3435 |
| 文献子类 | Article |
| 英文摘要 | From the venom of scorpion Buthus martensii Karsch, a short peptide (BmP01, 29 amino acid residues) was isolated and characterized as previously reported (Lebren, R., R., et al. (1997) Eur. J. Biochem. 245, 457-464). It was shown to reduce 33% outward HC channel (hippocampal neurons) currents at 10 mu M The solution structure of BmP01 was determined by 2D H-1 NMR spectroscopy. The NOEs, coupling constants, and H-D exchange obtained from NMR spectroscopy were used in structural calculations. The conformation of BmP01 is composed of a short alpha-helix (Cys 3-Thr 12) and a two-stranded antiparalleI beta-sheet (Ala IB-Asp 20 and Lys 23-Pro 28). There are three disulfide bridges (Cys S-Cys 19, Cys 6-Cys 24 and Cys 10-Cys 26) connecting the alpha-helix and beta-sheet. Asp 20 to Lys 23 form a type II turn linking the two strands. Structural and electrostatic potential comparison between BmP01 and its analogues are also presented. (C) 2000 Academic Press. |
| WOS关键词 | SENSITIVE POTASSIUM CHANNEL ; NUCLEAR-MAGNETIC-RESONANCE ; CA2+-ACTIVATED K+ CHANNELS ; LEIUROTOXIN-I ; BLOCKING PEPTIDE ; TOXINS ; CHARYBDOTOXIN ; PURIFICATION ; MACROMOLECULES ; NEUROTOXINS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000089794300052 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274565]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Wu, HM |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Acad Sinica, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, G,Li, YM,Wei, DS,et al. Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2000,276(3):1148-1154. |
| APA | Wu, G.,Li, YM.,Wei, DS.,He, FH.,Jiang, SK.,...&Wu, HM.(2000).Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,276(3),1148-1154. |
| MLA | Wu, G,et al."Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 276.3(2000):1148-1154. |
入库方式： OAI收割
来源：上海药物研究所
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