Comet electrophoresis of blood nucleated cells in genotoxicity assessment
文献类型：期刊论文
| 作者 | Guo, JY; Tu, ZH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1999-11 |
| 卷号 | 20期号:11页码:975-978 |
| 关键词 | agar gel electrophoresis L-4-oxalysine 10-hydroxycamptothecin norpregnenes dimethyl sulfoxide bleomycin mitomycin C lymphocytes cell survival |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: Genotoxicity evaluations of several different chemicals including L-4-oxalysine, 10-Hydroxycamptothecin (HCT), 19-norprogesteron( ST1435), dimethyl sulfoxide (Me2SO), bleomycin (BLM), and mitomycin C (MMC). METHODS: Alkaline comet assay in vitro (single cell gel) (SCG). RESULTS: L-4-oxalysine and HCT did not cause directly DNA damage. ST1435, the subdermal implant progestin, had no effect on DNA damage until the dose level up to 4 mmol.L-1. Me2SO did not increase DNA damage at concentration below 2 %, but showed a concentration-dependent DNA damage at greater than or equal to 4 %. Bleomycin and mitomycin C demonstrated a strong dose-dependent DNA damage. CONCLUSION: Comet assay as a tool to test the genotoxicity of suspected chemicals, is rapid, simple, sensitive, good reproducible, and inexpensive. |
| WOS关键词 | PROGESTIN ST-1435 |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000083583700003 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274678]  |
| 专题 | 药物安全性评价中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Tu, ZH |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, JY,Tu, ZH. Comet electrophoresis of blood nucleated cells in genotoxicity assessment[J]. ACTA PHARMACOLOGICA SINICA,1999,20(11):975-978. |
| APA | Guo, JY,&Tu, ZH.(1999).Comet electrophoresis of blood nucleated cells in genotoxicity assessment.ACTA PHARMACOLOGICA SINICA,20(11),975-978. |
| MLA | Guo, JY,et al."Comet electrophoresis of blood nucleated cells in genotoxicity assessment".ACTA PHARMACOLOGICA SINICA 20.11(1999):975-978. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。