The mechanism of epristeride against benign prostatic hyperplasia
文献类型：期刊论文
| 作者 | Sun, ZY; Wu, HY; Wang, MY; Tu, ZH |
| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 1999-04-29 |
| 卷号 | 371期号:2-3页码:227-233 |
| 关键词 | benign prostatic hyperplasia epristeride apoptosis cell culture finasteride (rat) |
| ISSN号 | 0014-2999 |
| DOI | 10.1016/S0014-2999(99)00109-0 |
| 文献子类 | Article |
| 英文摘要 | Epristeride, a 5 alpha-reductase inhibitor, decreases prostate size and improves symptoms in men with benign prostatic hyperplasia. However, little is known about the histopathology of the prostate after treatment with epristeride. To study the relationship between apoptosis and the mechanism of epristeride in the treatment of benign prostatic hyperplasia, the induction of apoptosis by epristeride was detected and measured in vitro by: (a) observing morphological changes in cells by light microscopy; (b) comparing the relative content of dihydrotestosterone in the rat prostate epithelial cells untreated and treated with epristeride by microspectrophotometry; (c) estimating changes in cell size and DNA integrity by flow cytometry; and (d) monitoring nucleosomal DNA fragmentation by agarose gel electrophoresis. The cells treated with epristeride showed a reduction in cell size, an increase in the cytoplasm/nuclear ratio, which is indicative of the condensation of nuclear chromatin, a significant decrease in optical density at 580 nm (OD580 nm), and an oligonucleosomal ladder and a subdiploid peak of DNA characteristic of apoptosis. Therefore, the mechanism of epristeride in the treatment of benign prostatic hyperplasia might be apoptosis stimulated by decreasing dihydrotestosterone level. (C) 1999 Elsevier Science B.V. All rights reserved. |
| WOS关键词 | STEROID 5-ALPHA-REDUCTASE ; INDUCED APOPTOSIS ; CELL-DEATH ; INHIBITOR ; FINASTERIDE ; GROWTH ; RATS |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000080209300014 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274732]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Sun, ZY |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, ZY,Wu, HY,Wang, MY,et al. The mechanism of epristeride against benign prostatic hyperplasia[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,1999,371(2-3):227-233. |
| APA | Sun, ZY,Wu, HY,Wang, MY,&Tu, ZH.(1999).The mechanism of epristeride against benign prostatic hyperplasia.EUROPEAN JOURNAL OF PHARMACOLOGY,371(2-3),227-233. |
| MLA | Sun, ZY,et al."The mechanism of epristeride against benign prostatic hyperplasia".EUROPEAN JOURNAL OF PHARMACOLOGY 371.2-3(1999):227-233. |
入库方式： OAI收割
来源：上海药物研究所
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