A K+ channel-blocking peptide from venom of Chinese scorpion Buthus martensii Karsch
文献类型：期刊论文
| 作者 | Wu, G; Wei, DS; He, FH; Hu, GY; Wu, HM |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1998-07 |
| 卷号 | 19期号:4页码:317-321 |
| 关键词 | scorpion venoms neurotoxins potassium channels patch-clamp techniques |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To purify and characterize a potassium channel blocker (BmP-3) from the venom of Chinese scorpion Buthus martensii Karsch. METHODS: (1) Purification was carried out by gel-filtration, cation-exchange, and reversed-phase chromatographies. N-terminal was directly sequenced by double-coupling manual method. Molecular weight was determined on an electrospray ionization mass spectrometer. Amino acid composition was analyzed after acidic hydrolysis for 20 h in HCl 6 mol.L-1 at 110 degrees C. (2) Toxicity tests were conducted in mice and cockroaches. (3) The inhibitory effects of BmP-3 on K+ channels were tested in acutely dissociated rat hippocampal pyramidal neurons using whole-cell patch-clamp configuration. RESULTS: (1) A pure peptide (BmP-3, 8.1 mg) was obtained, about 0.08 % of total proteins of the venom. The N-terminal sequences were VGCEE and the molecular weight was 2938 in ESI-mass spectra. (2) No death occurred at the dosage of 200 mu g in mice and 8 mu g in cockroaches. (3) The peptide at 10 mu mol.L-1 reduced the peak outward K+ currents by 63 % + 4 % in vitro. CONCLUSION: BmP-3 inhibited K+ channels. |
| WOS关键词 | LEIUROTOXIN-I ; TOXIN |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000074666500005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274819]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Wu, HM |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, G,Wei, DS,He, FH,et al. A K+ channel-blocking peptide from venom of Chinese scorpion Buthus martensii Karsch[J]. ACTA PHARMACOLOGICA SINICA,1998,19(4):317-321. |
| APA | Wu, G,Wei, DS,He, FH,Hu, GY,&Wu, HM.(1998).A K+ channel-blocking peptide from venom of Chinese scorpion Buthus martensii Karsch.ACTA PHARMACOLOGICA SINICA,19(4),317-321. |
| MLA | Wu, G,et al."A K+ channel-blocking peptide from venom of Chinese scorpion Buthus martensii Karsch".ACTA PHARMACOLOGICA SINICA 19.4(1998):317-321. |
入库方式： OAI收割
来源：上海药物研究所
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