Two-Armed Activation of Bone Mineral Deposition by the Flavones Baicalin and Baicalein, Encapsulated in Polyphosphate Microparticles
文献类型：期刊论文
| 作者 | Wang, Xiao-Hong1; Guo, Yue-Wei2 ; Tolba, Emad1; Kokkinopoulou, Maria3; Wiens, Matthias1; Schroeder, Heinz C.1; Mueller, Werner E. G.1
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| 刊名 | AMERICAN JOURNAL OF CHINESE MEDICINE
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| 出版日期 | 2017 |
| 卷号 | 45期号:3页码:533-555 |
| 关键词 | Baicalin Baicalein Polyphosphate Microparticles Bone Mineralization Primary Human Osteoblasts Calcium Efflux/Transport Phospholipase C Alkaline Phosphatase |
| ISSN号 | 0192-415X |
| DOI | 10.1142/S0192415X1750032X |
| 文献子类 | Article |
| 英文摘要 | In this study, we investigated the effect of the two flavonoids, baicalin (baicalein 7-O-beta-D-glucuronic acid) and its aglycone, baicalein (5,6,7-trihydroxyflavone), after encapsulation into amorphous calcium polyphosphate (Ca-polyP) microparticles on mineralization of primary human osteoblasts (phOSB). Both flavonoids, which come from root extracts of Scutellaria baicalensis Georgi, are used in Traditional Chinese Medicine, and are nontoxic in cells up to a concentration of 3 mu g/ml. The morphogenetically active, energy-rich Ca-polyP particles with a stoichiometric P: Ca ratio of 1: 2 are degraded by cellular alkaline phosphatase (ALP) to ortho-phosphate used for bone hydroxyapatite formation. Here we show that the flavone-loaded Ca-polyP microparticles are readily taken up by phOSB, resulting in the accumulation of polyP around the nuclei and the formation of intracellular vesicles containing the ALP. In addition, we demonstrate that baicalin/baicalein causes a rise of the intracellular calcium [Ca2+](i) a level which markedly is augmented after encapsulation into Ca-polyP, through activation of the phospholipase C. Moreover, both flavones, either alone or associated with Ca-polyP microparticles, upregulate the expression of the osteoblast calcium efflux channel, the plasma membrane Ca2+-ATPase (PMCA), while the expression of ALP, which promotes bone mineralization, is induced by Ca-polyP and by the flavones only if present in the Ca-polyP-microparticle-associated form. As a result, the extent of bone mineralization is markedly enhanced. Based on the two-armed activating function, new applications of baicalin/baicalein as a component of nutriceuticals for osteoporosis prevention or bone implants can be envisaged. |
| WOS关键词 | OSTEOBLAST-LIKE CELLS ; SCUTELLARIA-BAICALENSIS ; WNT/BETA-CATENIN ; SAOS-2 CELLS ; CULTURED OSTEOBLASTS ; SIGNALING PATHWAYS ; NA+/CA2+ EXCHANGE ; CALCIUM-PHOSPHATE ; PLASMA-MEMBRANE ; METABOLIC FUEL |
| 资助项目 | ERC[268476] ; Deutsche Forschungsgemeinschaft[Schr 277/10-3] ; European Commission[268476] ; European Commission[604036] ; European Commission[311848] ; International Human Frontier Science Program[00000000] ; BiomaTiCS research initiative of the University Medical Center, Mainz[00000000] |
| WOS研究方向 | Integrative & Complementary Medicine ; General & Internal Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000402314500008 |
| 出版者 | WORLD SCIENTIFIC PUBL CO PTE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275713]  |
| 专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Mueller, Werner E. G. |
| 作者单位 | 1.Johannes Gutenberg Univ Mainz, Univ Med Ctr, Inst Physiol Chem, ERC Adv Investigator Grant Res Grp, Duesbergweg 6, D-55128 Mainz, Germany; 2.Chinese Acad Sci, Shanghai Inst Materia Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Max Planck Inst Polymer Res, D-55128 Mainz, Germany |
| 推荐引用方式 GB/T 7714 | Wang, Xiao-Hong,Guo, Yue-Wei,Tolba, Emad,et al. Two-Armed Activation of Bone Mineral Deposition by the Flavones Baicalin and Baicalein, Encapsulated in Polyphosphate Microparticles[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2017,45(3):533-555. |
| APA | Wang, Xiao-Hong.,Guo, Yue-Wei.,Tolba, Emad.,Kokkinopoulou, Maria.,Wiens, Matthias.,...&Mueller, Werner E. G..(2017).Two-Armed Activation of Bone Mineral Deposition by the Flavones Baicalin and Baicalein, Encapsulated in Polyphosphate Microparticles.AMERICAN JOURNAL OF CHINESE MEDICINE,45(3),533-555. |
| MLA | Wang, Xiao-Hong,et al."Two-Armed Activation of Bone Mineral Deposition by the Flavones Baicalin and Baicalein, Encapsulated in Polyphosphate Microparticles".AMERICAN JOURNAL OF CHINESE MEDICINE 45.3(2017):533-555. |
入库方式： OAI收割
来源：上海药物研究所
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