Amelioration of salvianolic acid C on aortic structure in apolipoprotein E-deficient mice treated with angiotension II
文献类型：期刊论文
| 作者 | Wu, Peng1,2; Han, Na2; Yua, Haitao1; Wang, Linlin1; Li, Xue1; Dong, Zhihui3; Fu, Weiguo3; Yorinaka, Hoichi4; Cho, Kenka5; Wu, Wanying1
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| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 166页码:75-81 |
| 关键词 | Salvianolic acid C Aortic aneurysm Matrix metalloproteinase |
| ISSN号 | 0024-3205 |
| DOI | 10.1016/j.lfs.2016.09.012 |
| 文献子类 | Article |
| 英文摘要 | Aims: Aortic aneurysm is a disastrous vascular disease with high morbidity and mortality. Matrix metalloproteinases (MMPs), especially MMP-9, is implicated in the development of aortic aneurysm, but the effective MMP inhibitors are far from development. To develop new candidate compound for aortic aneurysm therapy, we evaluated the effects of salvianolic acid C (SalC) against the formation of aortic aneurysm. Materials and methods: Aortic aneurysm was induced by implantation of angiotension II (AngII) minipump in apolipoprotein E-deficient (ApoE(-/-)) mice. MMPs activity was evaluated by enzyme kinetic analysis in vitro and in-gel gelatin zymography in vivo. The formation of aortic aneurysm was confirmed based on aortic maximum diameter. Hematoxylin and eosin stain was used to evaluate aortic structure, picrosirius red stain was for collagen deposition, and orcein stain was for elastin fragmentation. Macrophage infiltration was detected by CD68 immunohistochemistry. Key findings: Firstly, SalC showed significant inhibition on the activity of MMP-2 and MMP-9. Aortic aneurysm was defined as >50% increase in maximum diameter of aorta, and the down-regulated tendency of 20 mg/kg SalC against formation of aortic aneurysm was detected. Also, 22.2% rupture was detected in ApoE(-/-) mice, while no rupture of aortic aneurysm was found with 20 mg/kg SalC treatment. Then, SalC was detected to maintain the integrity of aortic structure and protect elastin against fragmentation. Finally, SalC considerably inhibited infiltration of macrophage in the injury site of aorta. Significance: SalC significantly ameliorated the progression of aortic aneurysm in ApoE(-/-) mice, and held great potential for aortic aneurysm therapy. (C) 2016 Published by Elsevier Inc. |
| WOS关键词 | MATRIX METALLOPROTEINASES ; ANEURYSMS ; DOXYCYCLINE ; MATRIX-METALLOPROTEINASE-9 ; GROWTH |
| 资助项目 | Shanghai Science and Technology Development Foundation[14401900900] ; International Cooperation Bureau of the Chinese Academy of Sciences for Key Projects of Foreign Cooperation[Y6G8061024] ; National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2013ZX09103002-024] ; Croucher Foundation[CAS14201] ; State Key Laboratory of Drug Research[SIMM1501KF-13] |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000388101300012 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275787]  |
| 专题 | 上海中药现代化研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Yin, Jun; Jiang, Baohong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Shenyang Pharmaceut Univ, Wenhua Rd 103, Shenyang 110016, Peoples R China; 3.Fudan Univ, Zhongshan Hosp, Dept Vasc Surg, Shanghai, Peoples R China; 4.Kumamoto Univ, Grad Sch Med Sci, Dept Pharmacol & Mol Therapeut, 1-1-1 Honjo, Kumamoto, Japan; 5.Takarazuka Univ Med & Hlth Care, Takarazuka, Hyogo 6660162, Japan |
| 推荐引用方式 GB/T 7714 | Wu, Peng,Han, Na,Yua, Haitao,et al. Amelioration of salvianolic acid C on aortic structure in apolipoprotein E-deficient mice treated with angiotension II[J]. LIFE SCIENCES,2016,166:75-81. |
| APA | Wu, Peng.,Han, Na.,Yua, Haitao.,Wang, Linlin.,Li, Xue.,...&Jiang, Baohong.(2016).Amelioration of salvianolic acid C on aortic structure in apolipoprotein E-deficient mice treated with angiotension II.LIFE SCIENCES,166,75-81. |
| MLA | Wu, Peng,et al."Amelioration of salvianolic acid C on aortic structure in apolipoprotein E-deficient mice treated with angiotension II".LIFE SCIENCES 166(2016):75-81. |
入库方式： OAI收割
来源：上海药物研究所
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