Hypoglycemic Activity and the Potential Mechanism of the Flavonoid Rich Extract from Sophora tonkinensis Gagnep. in KK-Ay Mice
文献类型：期刊论文
| 作者 | Huang, Mi2; Deng, Shihao2; Han, Qianqian2; Zhao, Ping2; Zhou, Qi2; Zheng, Sijian2; Ma, Xinhua2; Xu, Chan2; Yang, Jing2; Yang, Xinzhou1,2,3 |
| 刊名 | FRONTIERS IN PHARMACOLOGY
 |
| 出版日期 | 2016-09-05 |
| 卷号 | 7 |
| 关键词 | hypoglycemic agents Sophora tonkinensis Gagnep. KK-Ay mice GLUT4 p-AMPK |
| ISSN号 | 1663-9812 |
| DOI | 10.3389/fphar.2016.00288 |
| 文献子类 | Article |
| 英文摘要 | This study investigated the active principles, hypoglycemic activity and potential mechanisms of the flavonoid rich extract from Sophora tonkinensis Gagnep. (ST-EtOAc) in KK-Ay diabetic mice. An off-line semipreparative liquid chromatography-nuclear magnetic resonance (LC-NMR) and liquid chromatography-ultraviolet-electrospray ionization mass spectrometry (LC-UV-ESIMS) protocol was performed to determine 13 flavonoids from ST-EtOAc. ST-EtOAc administrated orally to the KK-Ay mice significantly increased their sensibility to insulin, reduced fasting blood-glucose levels and blood lipid indexes such as triglyceride and cholesterol. Moreover, ST-EtOAc exhibited a strong effect of stimulation on glucose transporter 4 (GLUT4) translocation by 2.7-fold in L6 cells. However, the selective AMP-activated protein kinase (AMPK) inhibitor compound C can completely inhibit the activation of the AMPK pathway and prevent the GLUT4 translocation caused by ST-EtOAc. In vivo, phosphorylation of the AMPK expression in the liver and skeletal muscle was measured. The results showed phosphorylation of the AMPK had been improved and GLUT4 expression had been also enhanced. In this paper, we conclude that, ST-EtOAc seems to have potential beneficial effects on the treatment of type 2 diabetes mellitus with the probable mechanism of stimulating GLUT4 translocation modulated by the AMPK pathway. |
| WOS关键词 | PRENYLATED FLAVONOIDS ; GLUCOSE-TRANSPORTER ; SKELETAL-MUSCLE ; GLUT4 ; RATS ; CELL ; CONSTITUENTS ; DYSFUNCTION ; PATHWAY ; LEAVES |
| 资助项目 | National Natural Science Foundation of China[81573561] ; National Natural Science Foundation of China[81102798] ; National Natural Science Foundation of China[31070744] ; State Key Laboratory of Drug Research Foundation[SIMM1403KF-07] ; Open Research Program from the Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province[2015ZD004] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000382399400001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275899]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Yang, Xinzhou |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 2.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan, Peoples R China; 3.Tianjin Univ Sci & Technol, Coll Biol Engn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Mi,Deng, Shihao,Han, Qianqian,et al. Hypoglycemic Activity and the Potential Mechanism of the Flavonoid Rich Extract from Sophora tonkinensis Gagnep. in KK-Ay Mice[J]. FRONTIERS IN PHARMACOLOGY,2016,7. |
| APA | Huang, Mi.,Deng, Shihao.,Han, Qianqian.,Zhao, Ping.,Zhou, Qi.,...&Yang, Xinzhou.(2016).Hypoglycemic Activity and the Potential Mechanism of the Flavonoid Rich Extract from Sophora tonkinensis Gagnep. in KK-Ay Mice.FRONTIERS IN PHARMACOLOGY,7. |
| MLA | Huang, Mi,et al."Hypoglycemic Activity and the Potential Mechanism of the Flavonoid Rich Extract from Sophora tonkinensis Gagnep. in KK-Ay Mice".FRONTIERS IN PHARMACOLOGY 7(2016). |
入库方式： OAI收割
来源：上海药物研究所
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