Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells
文献类型：期刊论文
| 作者 | Morozumi, Yuichi2; Boussouar, Faycal2; Tan, Minjia3,4 ; Chaikuad, Apirat5,6; Jamshidikia, Mahya2; Colak, Gozde7; He, Huang7; Nie, Litong3,4; Petosa, Carlo8; de Dieuleveult, Maud1
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| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2016-08 |
| 卷号 | 8期号:4页码:349-362 |
| 关键词 | FACT epidrug germ cells cancer drug target histone turnover Pax3 histone chaperone |
| ISSN号 | 1674-2788 |
| DOI | 10.1093/jmcb/mjv060 |
| 文献子类 | Article |
| 英文摘要 | Although the conserved AAA ATPase and bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, using a combination of ChIP-seq, ChIP-proteomics, and RNA-seq experiments in embryonic stem cells where Atad2 is normally highly expressed, we found that Atad2 is an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication, and DNA repair factors. A structural analysis of its bromodomain and subsequent investigations demonstrate that histone acetylation guides ATAD2 to chromatin, resulting in an overall increase of chromatin accessibility and histone dynamics, which is required for the proper activity of the highly expressed gene fraction of the genome. While in exponentially growing cells Atad2 appears dispensable for cell growth, in differentiating ES cells Atad2 becomes critical in sustaining specific gene expression programmes, controlling proliferation and differentiation. Altogether, this work defines Atad2 as a facilitator of general chromatin-templated activities such as transcription. |
| WOS关键词 | HEPATOCELLULAR-CARCINOMA ; POOR-PROGNOSIS ; BROMODOMAIN ; PROTEIN ; EXPRESSION ; CANCER ; ANCCA ; IDENTIFICATION ; REPLICATION ; COACTIVATOR |
| 资助项目 | INCa[00000000] ; ANR EpiSperm2 project[00000000] ; foundation ARC[00000000] ; SGC, a registered charity[1097737] ; AbbVie[00000000] ; Bayer[00000000] ; Boehringer Ingelheim[00000000] ; Canada Foundation for Innovation[00000000] ; Canadian Institutes for Health Research[00000000] ; Genome Canada[00000000] ; GlaxoSmithKline[00000000] ; Janssen[00000000] ; Lilly Canada[00000000] ; Novartis Research Foundation[00000000] ; Ontario Ministry of Economic Development and Innovation[00000000] ; Pfizer[00000000] ; Takeda[00000000] ; Wellcome Trust[092809/Z/10/Z] ; European Union[278568 'PRIMES'] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5784173 |
| WOS记录号 | WOS:000384849900007 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275933]  |
| 专题 | 化学蛋白质组学研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Khochbin, Saadi |
| 作者单位 | 1.Univ Paris Saclay, Univ Paris Sud, Inst Integrat Biol Cell I2BC, CEA,CNRS,CEN Saclay, F-91191 Gif Sur Yvette, France; 2.Univ Grenoble Alpes, U823, Inst Albert Bonniot Grenoble, INSERM, F-38700 Grenoble, France; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 5.Univ Oxford, Nuffield Dept Clin Med, Struct Genom Consortium, Old Rd Campus Res Bldg,Roosevelt Dr, Oxford OX3 7DQ, England; 6.Univ Oxford, Nuffield Dept Clin Med, Target Discovery Inst TDI, NDM Res Bldg,Roosevelt Dr, Oxford OX3 7FZ, England; 7.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA; 8.Univ Grenoble Alpes, CNRS, Inst Biol Struct, CEA, F-38027 Grenoble, France; 9.Univ Lyon 1, ENS Lyon, Int Ctr Infectiol Res, CIRI,EVIR Team,INSERM,U1111,CNRS,UMR5308, Lyon, France; 10.INSERM, U1065, Ctr Mediterrane Med Mol C3M, Equipe Controle Metab Morts Cellulaires, F-06204 Nice, France; |
| 推荐引用方式 GB/T 7714 | Morozumi, Yuichi,Boussouar, Faycal,Tan, Minjia,et al. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2016,8(4):349-362. |
| APA | Morozumi, Yuichi.,Boussouar, Faycal.,Tan, Minjia.,Chaikuad, Apirat.,Jamshidikia, Mahya.,...&Khochbin, Saadi.(2016).Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells.JOURNAL OF MOLECULAR CELL BIOLOGY,8(4),349-362. |
| MLA | Morozumi, Yuichi,et al."Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells".JOURNAL OF MOLECULAR CELL BIOLOGY 8.4(2016):349-362. |
入库方式： OAI收割
来源：上海药物研究所
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