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Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition

文献类型：期刊论文

作者Zhang, Le-Le2; Xu, Yu-Lian2; Tang, Zheng-Hai2; Xu, Xiao-Huang2; Chen, Xin2; Li, Ting2; Ding, Chun-Yong3; Huang, Ming-Qing1; Chen, Xiu-Ping2; Wang, Yi-Tao2
刊名PHYTOMEDICINE
出版日期2016-07-15
卷号23期号:8页码:800-809
关键词Ovarian cancer Alisol B 23-acetate Cell cycle arrest Apoptosis Migration Invasion
ISSN号0944-7113
DOI10.1016/j.phymed.2016.04.003
文献子类Article
英文摘要Background: Ovarian cancer is the first leading cause of death among gynecologic malignancies worldwide. Discovery of new chemotherapeutic drugs is still imperative for the improvement of the survival rate. Purpose: This study aims to investigate the anti-cancer potential of alisol B 23-acetate (AB23), a protostane-type triterpene isolated from the Alismatis Rhizoma, in the parental and paclitaxel-resistant ovarian cancer cells. Methods: MTT assay was performed to evaluate cell viability after treatment with AB23, along with flow cytometry for apoptosis and cell cycle analysis. Western blotting was conducted to determine the relative protein level. Wound healing and transwell assays were performed to investigate the effect of AB23 on cell migration and invasion. Results: AB23 obviously inhibited proliferation of the three ovarian cancer cell lines, down-regulated the protein levels of CDK4, CDK6, and cyclin D1, and blocked the cell cycle progressions in G1 phase. Meanwhile, AB23 induced accumulation of the sub-G1 phase in the three cell lines in a concentration dependent manner. The protein levels of cleaved poly ADP-ribose polymerase (PARP) and the ratio of Bax/Bcl-2 were up-regulated after treatment with AB23. Further study showed that AB23 induced endoplasmic reticulum stress through IRE1 signaling pathway and silencing of IRE1 alpha partially enhanced AB23-induced apoptosis. Wound healing and transwell assays showed that AB23 could also suppress the migration and invasion of HEY cells. Moreover, it down-regulated the protein levels of matrix metalloproteinases MMP-2 and MMP-9. Conclusion: AB23 possessed anti-proliferation, anti-migration and anti-invasion activities as a single agent on ovarian cancer cells. (C) 2016 Elsevier GmbH. All rights reserved.
WOS关键词LUNG-CANCER ; MATRIX METALLOPROTEINASES ; ALISMATIS-RHIZOMA ; BREAST-CANCER ; METASTASIS ; TRITERPENE ; EXPRESSION ; ORIENTALIS ; RESISTANT ; RESPONSES
资助项目Science and Technology Development Fund, Macao S.A.R (FDCT)[038/2014/A1] ; Research Fund of University of Macau (One Time Special Fund for PhD Student in University of Macau)[MRG024-LJJ-2015-ICMS] ; Research Fund of University of Macau (One Time Special Fund for PhD Student in University of Macau)[MYRG2015-00091-ICMS-QRCM] ; Research Fund of University of Macau (One Time Special Fund for PhD Student in University of Macau)[MYRG2015-00101-ICMS-QRCM] ; Research Fund of University of Macau (One Time Special Fund for PhD Student in University of Macau)[CPG2014-00012-ICMS]
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
WOS记录号WOS:000377544000002
出版者ELSEVIER GMBH, URBAN & FISCHER VERLAG
源URL[http://119.78.100.183/handle/2S10ELR8/275958]  
专题药物化学研究室
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Yuan, Xiao-Feng; Lu, Jin-Jian
作者单位1.Fujian Univ Tradit Chinese Med, Coll Pharm, Fuzhou, Peoples R China;
2.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Ave Univ, Taipa, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China;
4.Zhejiang Chinese Med Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Le-Le,Xu, Yu-Lian,Tang, Zheng-Hai,et al. Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition[J]. PHYTOMEDICINE,2016,23(8):800-809.
APA Zhang, Le-Le.,Xu, Yu-Lian.,Tang, Zheng-Hai.,Xu, Xiao-Huang.,Chen, Xin.,...&Lu, Jin-Jian.(2016).Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition.PHYTOMEDICINE,23(8),800-809.
MLA Zhang, Le-Le,et al."Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migration and invasion inhibition".PHYTOMEDICINE 23.8(2016):800-809.

入库方式： OAI收割

来源：上海药物研究所

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