Analysis of diacylglycerols by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry: Double bond location and isomers separation
文献类型：期刊论文
| 作者 | Deng, Pan1; Zhong, Dafang1,2 ; Wang, Xi2; Dai, Yulu2; Zhou, Lei1; Leng, Ying2; Chen, Xiaoyan1
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| 刊名 | ANALYTICA CHIMICA ACTA
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| 出版日期 | 2016-06-21 |
| 卷号 | 925页码:23-33 |
| 关键词 | Diacylglycerol Liquid chromatography Mass spectrometry Dimethyl disulfide derivative Double bond location Collision cross-section |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2016.04.051 |
| 文献子类 | Article |
| 英文摘要 | Diacylglycerols (DAGs) are important lipid intermediates and have been implicated in human diseases. Isomerism complicates their mass spectrometric analysis; in particular, it is difficult to identify fatty acid substituents and locate the double bond positions in unsaturated DAGs. We have developed an analytical strategy using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) in conjunction with dimethyl disulfide (DMDS) derivatization and collision cross-section (CCS) measurement to characterize DAGs in biological samples. The method employs nonaqueous reversed-phase chromatographic separation and profile collision energy (CE) mode for MSE and MS/MS analyses. Three types of fragment ions were produced simultaneously. Hydrocarbon ions (m/z 50-200) obtained at high CE helped to distinguish unsaturated and saturated DAGs rapidly. Neutral loss ions and acylium ions (m/z 300-400) produced at low CE were used to identify fatty acid substituents. Informative methyl thioalkane fragment ions were used to locate the double bonds of unsaturated DAGs. Mono-methylthio derivatives were formed mainly by the reaction of DAGs with DMDS, where methyl thiol underwent addition to the first double bond farthest from the ester terminus of unsaturated fatty acid chains. The addition of CCS values maximized the separation of isomeric DAG species and improved the confidence of DAG identification. Fourteen DAGs were identified in mouse myotube cells based on accurate masses, characteristic fragment ions, DMDS derivatization, and CCS values. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | PROTEIN-KINASE-C ; FATTY-ACIDS ; ELECTROSPRAY-IONIZATION ; STRUCTURAL-CHARACTERIZATION ; INSULIN-RESISTANCE ; LITHIATED ADDUCTS ; ACYL-CHAIN ; ACTIVATION ; ALKYLTHIOLATION ; IDENTIFICATION |
| 资助项目 | Youth Innovation Promotion Association CAS[00000000] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000376130300003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275991]  |
| 专题 | 上海药物代谢研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Metab & Pharmacokinet Res, 501 Haike Rd, Shanghai 201210, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Pan,Zhong, Dafang,Wang, Xi,et al. Analysis of diacylglycerols by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry: Double bond location and isomers separation[J]. ANALYTICA CHIMICA ACTA,2016,925:23-33. |
| APA | Deng, Pan.,Zhong, Dafang.,Wang, Xi.,Dai, Yulu.,Zhou, Lei.,...&Chen, Xiaoyan.(2016).Analysis of diacylglycerols by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry: Double bond location and isomers separation.ANALYTICA CHIMICA ACTA,925,23-33. |
| MLA | Deng, Pan,et al."Analysis of diacylglycerols by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry: Double bond location and isomers separation".ANALYTICA CHIMICA ACTA 925(2016):23-33. |
入库方式： OAI收割
来源：上海药物研究所
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