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Global Profiling of Protein Lysine Malonylation in Escherichia coli Reveals Its Role in Energy Metabolism

文献类型：期刊论文

作者Qian, Lili2,3,4; Nie, Litong2,3,4; Chen, Ming2,3,4; Liu, Ping2,3,4; Zhu, Jun5; Zhai, Linhui2,3,4; Tao, Sheng-ce1; Cheng, Zhongyi5; Zhao, Yingming2,3,4,6; Tan, Minjia2,3,4
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2016-06
卷号15期号:6页码:2060-2071
关键词lysine malonylation Escherichia coli affinity enrichment mass spectrometry protein post-translational modification (PTM) energy metabolism
ISSN号1535-3893
DOI10.1021/acs.jproteome.6b00264
文献子类Article
英文摘要Protein lysine malonylation is a recently identified post-translational modification (PTM), which is evolutionarily conserved from bacteria to mammals. Although analysis of lysine malonylome in mammalians suggested that this modification was related to energy metabolism, the substrates and biological roles of malonylation in prokaryotes are still poorly understood. In this study, we performed qualitative and quantitative analyses to globally identify lysine malonylation substrates in Escherichia coli. We identified 1745 malonylation sites in 594 proteins in E. coli, representing the first and largest malonylome data set in prokaryotes up to date. Bioinformatic analyses showed that lysine malonylation was significantly enriched in protein translation, energy metabolism pathways and fatty acid biosynthesis, implying the potential roles of protein malonylation in bacterial physiology. Quantitative proteomics by fatty acid synthase inhibition in both auxotrophic and prototrophic E. coli strains revealed that lysine malonylation is closely associated with E. coli fatty acid metabolism. Protein structural analysis and mutagenesis experiment suggested malonylation could impact enzymatic activity of citrate synthase, a key enzyme in citric acid (TCA) cycle. Further comparative analysis among lysine malonylome, succinylome and acetylome data showed that these three modifications could participate in some similar enriched metabolism pathways, but they could also possibly play distinct roles such as in fatty acid synthesis. These data expanded our knowledge of lysine malonylation in prokaryotes, providing a resource for functional study of lysine malonylation in bacteria.
WOS关键词POSTTRANSLATIONAL MODIFICATION ; INTERACTION NETWORKS ; ACETYLATION ; SUCCINYLATION ; IDENTIFICATION ; SIRT5 ; GLUTARYLATION ; COMPLEXES ; MODELS ; FLUX
资助项目National Basic Research Program of China (973 Program)[2014CBA02004] ; Natural Science Foundation of China[31370814] ; Natural Science Foundation of China[31370813] ; National Science AMP[00000000] ; Technology Major Project[2014ZX09507-002] ; Shanghai Municipal Science and Technology Commission[14DZ2261100] ; Shanghai Municipal Science and Technology Commission[15410723100] ; National Institute of Health (NIH) of United States[GM105933] ; National Institute of Health (NIH) of United States[DK107868] ; National Institute of Health (NIH) of United States[GM115961]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000377319200028
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/276025]  
专题化学蛋白质组学研究中心
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Tan, Minjia
作者单位1.Shanghai Jiao Tong Univ, Minist Educ, Key Lab Syst Biomed, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 201203, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
5.Jingjie PTM BioLab Hangzhou Co Ltd, Hangzhou 310018, Peoples R China;
6.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA
推荐引用方式
GB/T 7714		 Qian, Lili,Nie, Litong,Chen, Ming,et al. Global Profiling of Protein Lysine Malonylation in Escherichia coli Reveals Its Role in Energy Metabolism[J]. JOURNAL OF PROTEOME RESEARCH,2016,15(6):2060-2071.
APA Qian, Lili.,Nie, Litong.,Chen, Ming.,Liu, Ping.,Zhu, Jun.,...&Tan, Minjia.(2016).Global Profiling of Protein Lysine Malonylation in Escherichia coli Reveals Its Role in Energy Metabolism.JOURNAL OF PROTEOME RESEARCH,15(6),2060-2071.
MLA Qian, Lili,et al."Global Profiling of Protein Lysine Malonylation in Escherichia coli Reveals Its Role in Energy Metabolism".JOURNAL OF PROTEOME RESEARCH 15.6(2016):2060-2071.

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来源：上海药物研究所

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