A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1
文献类型：期刊论文
| 作者 | Miao, Lingling2; Yao, Hailan1,4; Li, Chenggang2; Pu, Mengfan2; Yao, Xuan1; Yang, Hui1; Qi, Xinming2 ; Ren, Jin2; Wang, Yizheng3
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| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
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| 出版日期 | 2016-04 |
| 卷号 | 1859期号:4页码:650-662 |
| 关键词 | MicroRNA Cytochrome P450 2E1 Gene regulation Transcription Translation |
| ISSN号 | 1874-9399 |
| DOI | 10.1016/j.bbagrm.2016.02.016 |
| 文献子类 | Article |
| 英文摘要 | MicroRNAs (miRNAs) can direct post-transcriptional or transcriptional gene silencing. Here, we report that miR-552 is in the nucleus and cytosol and inhibits human cytochrome P450 (CYP) 2E1 expression at both transcriptional and post-transcriptional levels. MiR-552 via its non-seed sequence forms hybrids with a loop hairpin of the cruciform structure in CYP2E1 promoter region to inhibit SMARCE1 and RNA polymerase II binding to the promoter and CYP2E1 transcription. Expressing SMARCE1 reverses the inhibitory effects of miR-552 on CYP2E1 mRNA expression. MiR-552 with mutations in non-seed region losses its transcriptional, but retains its post transcriptional repression to CYP2E1. In contrast, mutation in miR-552 seed sequence suppresses its inhibitory effects on CYP2E1 expression at protein, but not at mRNA, levels. Our results suggest that miR-552 is a miRNA with a dual inhibitory ability at transcriptional and post-transcriptional levels leading to an effective inhibition. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | INDUCED LIVER-INJURY ; BREAST-CANCER CELLS ; POSTTRANSCRIPTIONAL REGULATION ; CYP2E1 EXPRESSION ; MAMMALIAN-CELLS ; SWI/SNF COMPLEX ; DNA METHYLATION ; GENE PROMOTERS ; TARGET ; PROTEIN |
| 资助项目 | National Natural Science Foundation of China[81] ; National Natural Science Foundation of China[130] ; National Natural Science Foundation of China[081] ; National Science and Technology Major Project[2012ZX09302-003] ; National Science and Technology Major Project[2015ZX09102005] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000374370500011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276087]  |
| 专题 | 药物安全性评价中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ren, Jin; Wang, Yizheng |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neuroscience,State Key Lab Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Beijing Inst Basic Med Sci, Brain Sci Ctr, 27 Taiping Rd, Beijing 100850, Peoples R China; 4.Roskamp Inst, Ctr Adv Therapeut Strategies Brain Disorders, Sarasota, FL 34243 USA |
| 推荐引用方式 GB/T 7714 | Miao, Lingling,Yao, Hailan,Li, Chenggang,et al. A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,2016,1859(4):650-662. |
| APA | Miao, Lingling.,Yao, Hailan.,Li, Chenggang.,Pu, Mengfan.,Yao, Xuan.,...&Wang, Yizheng.(2016).A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1.BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,1859(4),650-662. |
| MLA | Miao, Lingling,et al."A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1".BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS 1859.4(2016):650-662. |
入库方式： OAI收割
来源：上海药物研究所
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