G protein-coupled receptor GPR160 is associated with apoptosis and cell cycle arrest of prostate cancer cells
文献类型：期刊论文
| 作者 | Zhou, Caihong2,3; Dai, Xinchuan2,3; Chen, Yi4 ; Shen, Yanyan4; Lei, Saifei2,3; Xiao, Ting2,3; Bartfai, Tamas1; Ding, Jian4; Wang, Ming-Wei2,3,5
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| 刊名 | ONCOTARGET
 |
| 出版日期 | 2016-03-15 |
| 卷号 | 7期号:11页码:12823-12839 |
| ISSN号 | 1949-2553 |
| 关键词 | GPR160 prostate cancer orphan G protein-coupled receptor cell cycle arrest apoptosis |
| DOI | 10.18632/oncotarget.7313 |
| 文献子类 | Article |
| 英文摘要 | G protein-coupled receptors (GPCRs) represent the largest membrane protein family implicated in the therapeutic intervention of a variety of diseases including cancer. Exploration of biological actions of orphan GPCRs may lead to the identification of new targets for drug discovery. This study investigates potential roles of GPR160, an orphan GPCR, in the pathogenesis of prostate cancer. The transcription levels of GPR160 in the prostate cancer tissue samples and cell lines, such as PC-3, LNCaP, DU145 and 22Rv1 cells, were significantly higher than that seen in normal prostate tissue and cells. Knockdown of GPR160 by lentivirus-mediated short hairpin RNA constructs targeting human GPR160 gene (ShGPR160) resulted in prostate cancer cell apoptosis and growth arrest both in vitro and in athymic mice. Differential gene expression patterns in PC-3 cells infected with ShGPR160 or scramble lentivirus showed that 815 genes were activated and 1193 repressed. Functional annotation of differentially expressed genes (DEGs) revealed that microtubule cytoskeleton, cytokine activity, cell cycle phase and mitosis are the most evident functions enriched by the repressed genes, while regulation of programmed cell death, apoptosis and chemotaxis are enriched significantly by the activated genes. Treatment of cells with GPR160-targeting shRNA lentiviruses or duplex siRNA oligos increased the transcription of IL6 and CASP1 gene significantly. Our data suggest that the expression level of endogenous GPR160 is associated with the pathogenesis of prostate cancer. |
| WOS关键词 | ANDROGEN RECEPTOR ; DRUG TARGETS ; EXPRESSION ; GPCRS ; GENE ; IMMORTALIZATION ; PROGRESSION ; ACTIVATION ; CASPASE-1 ; MICRORNA |
| 资助项目 | National Health and Family Planning Commission[2012ZX09304-011] ; National Health and Family Planning Commission[2013ZX09401003-005] ; National Health and Family Planning Commission[2013ZX09507001] ; National Health and Family Planning Commission[2013ZX09507-002] ; Shanghai Science and Technology Development Fund[15DZ2291600] ; Shanghai Natural Science Foundation[13ZR1410700] ; Thousand Talents Program in China[00000000] |
| WOS研究方向 | Oncology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | IMPACT JOURNALS LLC |
| WOS记录号 | WOS:000375679600081 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276101]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Wang, Ming-Wei |
| 作者单位 | 1.Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 5.Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Caihong,Dai, Xinchuan,Chen, Yi,et al. G protein-coupled receptor GPR160 is associated with apoptosis and cell cycle arrest of prostate cancer cells[J]. ONCOTARGET,2016,7(11):12823-12839. |
| APA | Zhou, Caihong.,Dai, Xinchuan.,Chen, Yi.,Shen, Yanyan.,Lei, Saifei.,...&Wang, Ming-Wei.(2016).G protein-coupled receptor GPR160 is associated with apoptosis and cell cycle arrest of prostate cancer cells.ONCOTARGET,7(11),12823-12839. |
| MLA | Zhou, Caihong,et al."G protein-coupled receptor GPR160 is associated with apoptosis and cell cycle arrest of prostate cancer cells".ONCOTARGET 7.11(2016):12823-12839. |
入库方式： OAI收割
来源：上海药物研究所
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