A novel ultra-performance liquid chromatography hyphenated with quadrupole time of flight mass spectrometry method for rapid estimation of total toxic retronecine-type of pyrrolizidine alkaloids in herbs without requiring corresponding standards
文献类型：期刊论文
| 作者 | Zhu, Lin1,2; Ruan, Jian-Qing1,2; Li, Na4; Fu, Peter P.3; Ye, Yang2,5 ; Lin, Ge1,2
|
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 194页码:1320-1328 |
| 关键词 | Food safety Hepatotoxic Pyrrolizidine alkaloids Retronecine type UHPLC-MS |
| ISSN号 | 0308-8146 |
| DOI | 10.1016/j.foodchem.2014.11.093 |
| 文献子类 | Article |
| 英文摘要 | Nearly 50% of naturally-occurring pyrrolizidine alkaloids (PAs) are hepatotoxic, and the majority of hepatotoxic PM are retronecine-type PM (RET-PAs). However, quantitative measurement of PM in herbs/foodstuffs is often difficult because most of reference PM are unavailable. In this study, a rapid, selective, and sensitive UHPLC-QTOF-MS method was developed for the estimation of RET-PAs in herbs without requiring corresponding standards. This method is based on our previously established characteristic and diagnostic mass fragmentation patterns and the use of retrorsine for calibration. The use of a single RET-PA retrorsine) for construction of calibration was based on high similarities with no significant differences demonstrated by the calibration curves constructed by peak areas of extract ion chromatograms of fragment ion at m/z 120.0813 or 138.0919 versus concentrations of five representative RET-PAs. The developed method was successfully applied to measure a total content of toxic RET-PAs of diversified structures in fifteen potential PA-containing herbs. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS关键词 | N-OXIDES ; METABOLIC-ACTIVATION ; SENECIO-SCANDENS ; DNA-ADDUCTS ; HPLC-MS ; PLANTS ; TUMORIGENICITY ; IDENTIFICATION ; QIANLIGUANG ; INTERFACE |
| 资助项目 | Research Grant Council of Hong Kong (GRF)[471013] ; Research Grant Council of Hong Kong (GRF)[469712] |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Nutrition & Dietetics |
| 语种 | 英语 |
| WOS记录号 | WOS:000364248900170 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276132]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Lin, Ge |
| 作者单位 | 1.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Hong Kong, Hong Kong, Peoples R China; 2.Chinese Univ Hong Kong, Chinese Acad Sci, Shanghai Inst Mat Med, Joint Res Lab Promoting Globalizat Tradit Chinese, Hong Kong, Hong Kong, Peoples R China; 3.Natl Ctr Toxicol Res, Jefferson, AR 72079 USA; 4.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau Inst Appl Res Med & Hlth, Taipa, Macau, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Chem, State Key Lab Drug Res & Nat Prod, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Lin,Ruan, Jian-Qing,Li, Na,et al. A novel ultra-performance liquid chromatography hyphenated with quadrupole time of flight mass spectrometry method for rapid estimation of total toxic retronecine-type of pyrrolizidine alkaloids in herbs without requiring corresponding standards[J]. FOOD CHEMISTRY,2016,194:1320-1328. |
| APA | Zhu, Lin,Ruan, Jian-Qing,Li, Na,Fu, Peter P.,Ye, Yang,&Lin, Ge.(2016).A novel ultra-performance liquid chromatography hyphenated with quadrupole time of flight mass spectrometry method for rapid estimation of total toxic retronecine-type of pyrrolizidine alkaloids in herbs without requiring corresponding standards.FOOD CHEMISTRY,194,1320-1328. |
| MLA | Zhu, Lin,et al."A novel ultra-performance liquid chromatography hyphenated with quadrupole time of flight mass spectrometry method for rapid estimation of total toxic retronecine-type of pyrrolizidine alkaloids in herbs without requiring corresponding standards".FOOD CHEMISTRY 194(2016):1320-1328. |
入库方式： OAI收割
来源：上海药物研究所
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