Discovery of a novel fluorescent probe for the sensitive detection of beta-amyloid deposits
文献类型：期刊论文
| 作者 | Ren, Wenming3; Xu, Mingming4; Liang, Steven H.1,5; Xiang, Huaijiang2; Tang, Li2; Zhang, Minkui2; Ding, Dejun3; Li, Xin2; Zhang, Haiyan4 ; Hu, Youhong2,3
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2016-01-15 |
| 卷号 | 75页码:136-141 |
| 关键词 | Fluorescent probe Imaging A beta plaques Alzheimer's disease Photoinduced electron transfer |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2015.08.030 |
| 文献子类 | Article |
| 英文摘要 | Here we reported the development of the first photoinduced electron transfer (PeT) probe (1) to directly locate beta-amyloid aggregates (A beta plaques) in the brain without the need of post-washing procedures. The probe showed a high affinity for A beta aggregates with a K-d value of 3.5 nM. It is weakly emissive by itself with its fluorescence quenched by electron transfer from PeT donor to the excited fluorophore. But selective binding to A beta plaques would attenuate the PeT process and restore the fluorescence, therefore facilitating the tracking of A beta plaques. The probe is advantageous in that its fluorescence is environmentless-sensitive and no washing procedure is required to provide high contrast fluorescent signal when applied to stain brain tissues. As a proof of concept, its application has been exemplified by staining A beta plaques in slices of brain tissue from double transgenic (APP/PS1) mice of Alzheimer's disease. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | IN-VIVO DETECTION ; PHOTOINDUCED ELECTRON-TRANSFER ; ALZHEIMERS-DISEASE ; RECENT PROGRESS ; MOUSE-BRAIN ; PLAQUES ; DESIGN ; PROTEIN ; DERIVATIVES ; HYPOTHESIS |
| 资助项目 | National Natural Science Foundation of China[81225022] ; National Natural Science Foundation of China[21172232] |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000362862000020 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276178]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物化学研究室 |
| 通讯作者 | Li, Xin |
| 作者单位 | 1.Massachusetts Gen Hosp, Div Nucl Med & Mol Imaging, Boston, MA 02114 USA; 2.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310027, Zhejiang, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 200031, Peoples R China; 5.Harvard Univ, Sch Med, Dept Radiol, Boston, MA 02114 USA |
| 推荐引用方式 GB/T 7714 | Ren, Wenming,Xu, Mingming,Liang, Steven H.,et al. Discovery of a novel fluorescent probe for the sensitive detection of beta-amyloid deposits[J]. BIOSENSORS & BIOELECTRONICS,2016,75:136-141. |
| APA | Ren, Wenming.,Xu, Mingming.,Liang, Steven H..,Xiang, Huaijiang.,Tang, Li.,...&Hu, Youhong.(2016).Discovery of a novel fluorescent probe for the sensitive detection of beta-amyloid deposits.BIOSENSORS & BIOELECTRONICS,75,136-141. |
| MLA | Ren, Wenming,et al."Discovery of a novel fluorescent probe for the sensitive detection of beta-amyloid deposits".BIOSENSORS & BIOELECTRONICS 75(2016):136-141. |
入库方式： OAI收割
来源：上海药物研究所
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