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Efficient separation of curcumin, demethoxycurcumin, and bisdemethoxycurcumin from turmeric using supercritical fluid chromatography: From analytical to preparative scale

文献类型：期刊论文

作者Song, Wei3; Qiao, Xue3; Liang, Wen-fei3; Ji, Shuai3; Yang, Lu3; Wang, Yuan3; Xu, Yong-wei1; Yang, Ying1; Guo, De-an2,3; Ye, Min2,3
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2015-10
卷号38期号:19页码:3450-3453
关键词Bisdemethoxycurcumin Curcumin Curcuma longa L Demethoxycurcumin Supercritical fluid chromatography
ISSN号1615-9306
DOI10.1002/jssc.201500686
文献子类Article
英文摘要Curcumin is the major constituent of turmeric (Curcuma longa L.). It has attracted widespread attention for its anticancer and anti-inflammatory activities. The separation of curcumin and its two close analogs, demethoxycurcumin and bisdemethoxycurcumin, has been challenging by conventional techniques. In this study, an environmentally friendly method based on supercritical fluid chromatography was established for the rapid and facile separation of the three curcuminoids directly from the methanol extract of turmeric. The method was first developed and optimized by ultra performance convergence chromatography, and was then scaled up to preparative supercritical fluid chromatography. Eluted with supercritical fluid CO2 containing 8-15% methanol (containing 10mM oxalic acid) at a flow rate of 80 mL/min, curcumin, demethoxycurcumin and bisdemethoxycurcumin could be well separated on a Viridis BEH OBD column (Waters, 250 mm x 19 mm, 5 mu m) within 6.5 min. As a result, 20.8 mg of curcumin (97.9% purity), 7.0 mg of demethoxycurcumin (91.1%), and 4.6 mg of bisdemethoxycurcumin (94.8%) were obtained after a single step of supercritical fluid chromatography separation with a mean recovery of 76.6%. Showing obvious advantages in low solvent consumption, large sample loading, and easy solvent removal, supercritical fluid chromatography was proved to be a superior technique for the efficient separation of natural products.
WOS关键词PHASE LIQUID-CHROMATOGRAPHY ; STATIONARY-PHASE ; PURIFICATION ; EXTRACTION ; EXPRESSION ; MOLECULE ; RHIZOMES ; PATHWAY ; KINASE
资助项目National Natural Science Foundation of China[81222054] ; National Natural Science Foundation of China[81470172] ; Program for New Century Excellent Talents in University from Chinese Ministry of Education[NCET-11-0019] ; State Key Laboratory of Drug Research[SIMM1403KF-16]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000362553600019
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/276381]  
专题上海中药现代化研究中心
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Ye, Min
作者单位1.Waters Corp, Shanghai, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China;
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China;
推荐引用方式
GB/T 7714		 Song, Wei,Qiao, Xue,Liang, Wen-fei,et al. Efficient separation of curcumin, demethoxycurcumin, and bisdemethoxycurcumin from turmeric using supercritical fluid chromatography: From analytical to preparative scale[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(19):3450-3453.
APA Song, Wei.,Qiao, Xue.,Liang, Wen-fei.,Ji, Shuai.,Yang, Lu.,...&Ye, Min.(2015).Efficient separation of curcumin, demethoxycurcumin, and bisdemethoxycurcumin from turmeric using supercritical fluid chromatography: From analytical to preparative scale.JOURNAL OF SEPARATION SCIENCE,38(19),3450-3453.
MLA Song, Wei,et al."Efficient separation of curcumin, demethoxycurcumin, and bisdemethoxycurcumin from turmeric using supercritical fluid chromatography: From analytical to preparative scale".JOURNAL OF SEPARATION SCIENCE 38.19(2015):3450-3453.

入库方式： OAI收割

来源：上海药物研究所

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