Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry
文献类型：期刊论文
| 作者 | Qiao, Xue1; Song, Wei1; Ji, Shuai1; Wang, Qi1; Guo, De-an1,2 ; Ye, Min1,2
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2015-07-10 |
| 卷号 | 1402页码:36-45 |
| 关键词 | 2DLC Licorice Glycyrrhiza uralensis Phenolic compounds Triterpenoid saponins |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2015.05.006 |
| 文献子类 | Article |
| 英文摘要 | Licorice is one of the most popular herbal medicines worldwide. It contains a big array of phenolic compounds (flavonoids, coumarins, and diphenylethanones). Due to high structural diversity, low abundance, and co-elution with licorice saponins, these phenolic compounds are difficult to be separated by conventional chromatography. In this study, a mobile phase-dependent reversed-phase x reversed phase comprehensive two-dimensional liquid chromatography (RP x RP 2DLC) method was established to separate phenolic compounds in licorice (the roots of Glycyrrhiza uralensis). Organic solvents in the mobile phase were optimized to improve orthogonality of the first and second dimensions, and a synchronized gradient mode was used to improve chromatographic resolution. Finally, licorice extracts were eluted with methanol/water/formic acid in the first dimension (Acquity CSH C18 column), and acetonitrile/water/formic acid in the second dimension (Poroshell Phenyl-Hexyl column). By using this 2DLC system, a total of 311 compounds were detected within 40 min. The practical and effective peak capacity was 1329 and 524, respectively, and the orthogonality was 79.8%. The structures of 21 selected unknown compounds were tentatively characterized by mass spectrometry, and 8 of them were discovered from G. uralensis for the first time. The mobile phase-dependent 2DLC/MS system could benefit the separation and characterization of natural products in complicated herbal extracts. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | PRENYLATED FLAVONOIDS ; ACTIVE COMPONENTS ; CONSTITUENTS ; ROOTS ; 2ND-DIMENSION ; GLABRA ; ASSAY |
| 资助项目 | National Natural Science Foundation of China[81222054] ; National Natural Science Foundation of China[81470172] ; Program for New Century Excellent Talents in University from Chinese Ministry of Education[NCET-11-0019] ; State Key Laboratory of Drug Research[SIMM1403KF-16] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000356979900005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276469]  |
| 专题 | 上海中药现代化研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ye, Min |
| 作者单位 | 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Xue,Song, Wei,Ji, Shuai,et al. Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1402:36-45. |
| APA | Qiao, Xue,Song, Wei,Ji, Shuai,Wang, Qi,Guo, De-an,&Ye, Min.(2015).Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1402,36-45. |
| MLA | Qiao, Xue,et al."Separation and characterization of phenolic compounds and triterpenoid saponins in licorice (Glycyrrhiza uralensis) using mobile phase-dependent reversed-phase x reversed-phase comprehensive two-dimensional liquid chromatography coupled with mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1402(2015):36-45. |
入库方式： OAI收割
来源：上海药物研究所
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