In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis
文献类型：期刊论文
| 作者 | Wang, Jianhao2; Li, Jingyan2; Li, Jinchen2; Qin, Yuqin2; Wang, Cheli2; Qiu, Lin2; Jiang, Pengju1,2 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2015-07 |
| 卷号 | 36期号:14页码:1523-1528 |
| 关键词 | CE-FL In-capillary Protein Self-assembly Quantum dots |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201500073 |
| 文献子类 | Article |
| 英文摘要 | As a vast number of novel materials in particular inorganic nanoparticles have been invented and introduced to all aspects of life, public concerns about how they might affect our ecosystem and human life continue to arise. Such incertitude roots at a fundamental question of how inorganic nanoparticles self-assemble with biomolecules in solution. Various techniques have been developed to probe the interaction between particles and biomolecules, but very few if any can provide advantages of both rapid and convenient. Herein, we report a systematic investigation on quantum dots (QDs) and protein self-assembly inside a capillary. QDs and protein were injected to a capillary one after another. They were mixed inside the capillary when a high voltage was applied. Online separation and detection were then achieved. This new method can also be used to study the self-assembly kinetics of QDs and protein using the Hill equation, the K-D value for the self-assembly of QDs and protein was calculated to be 8.8 M. The obtained results were compared with the previous out of-capillary method and confirmed the effectiveness of the present method. |
| WOS关键词 | PEPTIDE ; CDTE ; NANOCRYSTALS ; KINETICS ; CDSE |
| 资助项目 | National High Technology Research and Development Program of China (863 Program)[2014AA020521] ; National Natural Science Foundation of China[81201085] ; National Natural Science Foundation of China[81473269] ; Natural Science Foundation of Jiangsu Province[BK20141170] ; Science & Technology Support Program of Changzhou (Society Development)[CE20145022] ; Priority Academic Program Development of Jiangsu Higher Education Institutions[00000000] ; State Key Laboratory of Drug Research[SIMM1501KF-08] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000358010400001 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276477]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Jiang, Pengju |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Changzhou Univ, Sch Pharmaceut Engn & Life Sci, Changzhou 213164, Jiangsu, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Jianhao,Li, Jingyan,Li, Jinchen,et al. In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis[J]. ELECTROPHORESIS,2015,36(14):1523-1528. |
| APA | Wang, Jianhao.,Li, Jingyan.,Li, Jinchen.,Qin, Yuqin.,Wang, Cheli.,...&Jiang, Pengju.(2015).In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis.ELECTROPHORESIS,36(14),1523-1528. |
| MLA | Wang, Jianhao,et al."In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis".ELECTROPHORESIS 36.14(2015):1523-1528. |
入库方式： OAI收割
来源：上海药物研究所
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