Platycodin D triggers autophagy through activation of extracellular signal-regulated kinase in hepatocellular carcinoma HepG2 cells
文献类型：期刊论文
| 作者 | Li, Ting1; Tang, Zheng-Hai1; Xu, Wen-Shan1; Wu, Guo-Sheng1; Wang, Ya-Fang1; Chang, Lin-Lin2; Zhu, Hong2; Chen, Xiu-Ping1; Wang, Yi-Tao1; Chen, Yi
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| 刊名 | EUROPEAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2015-02-15 |
| 卷号 | 749页码:81-88 |
| 关键词 | Platycodin D Auto phagy ERK Chloroquine Protective HepG2 |
| ISSN号 | 0014-2999 |
| DOI | 10.1016/j.ejphar.2015.01.003 |
| 文献子类 | Article |
| 英文摘要 | Platycodin D (PD), isolated from the Chinese medicinal herb named Platycodonis Radix, is a triterpenoid saponin with well-known anti tumor effects. In this study, we provided reliable evidence that PD triggered autophagy in a number of cell lines in vitro. PD triggered autophagy was identified by observation of cytoplasmic vacuole, up regulation of microtubule-associated protein 1 light chain 3 II (LC3-II), and accumulation of autophagosomes. The Akt/mammalian target of rapamycin (mTOR) pathway may be not involved in PD triggered autophagy, as evidenced by the increased phosphorylation of Akt (Thr308), mTOR (Ser2448), ribosomal protein S6 kinase (Ser371), and ULK1 (Ser757). However, the exlracellular signal regulated kinase (ERR) was activated after PD treatment. The decreased ERR phosphorylarion caused by pretreatment with U0126, an inhibitor of MEK, suppressed the expression of LC3-II compared with PD treatment alone, suggesting that ERR pathway may have a critical function in PD triggered autophagy. In addition, the PD-induced proliferative inhibition and apoptosis were enhanced when pretreatment with aulophagy inhibitor chloroquine (CQ) or bafilomycin A1 (BAF), indicating that PD may trigger a protective aulophagy in HepG2 cells. To the best of our knowledge, this paper is the first to report that PD triggers autophagy in a series of cell lines and ERR activation is important for PD-triggered autophagy in hepatocellular carcinoma HepG2 cells. The combined treatment with PD and CQ or BAF may be a promising regimen for hepatocellular carcinoma treatment. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | D INDUCES APOPTOSIS ; CANCER CELLS ; TRITERPENOID SAPONIN ; ELECTRON-MICROSCOPY ; MEDIATED APOPTOSIS ; LEUKEMIA-CELLS ; DEATH ; PATHWAY ; MTOR ; GRANDIFLORUM |
| 资助项目 | Macao Science and Technology Development Fund[070/2013/A] ; University of Macau[MRG008-LJJ2014-ICMS] ; University of Macau[MRG013-WYT-2013-ICMS] ; State Key Laboratory of Drug Research[SIMM1403KF-11] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000349761800010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276638]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Li, Ting |
| 作者单位 | 1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China; 2.Zhejiang Univ, Coll Pharmaceut Sci, Zhejiang Prov Key Lab Anticanc Drug Res, Hangzhou, Zhejiang, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ting,Tang, Zheng-Hai,Xu, Wen-Shan,et al. Platycodin D triggers autophagy through activation of extracellular signal-regulated kinase in hepatocellular carcinoma HepG2 cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2015,749:81-88. |
| APA | Li, Ting.,Tang, Zheng-Hai.,Xu, Wen-Shan.,Wu, Guo-Sheng.,Wang, Ya-Fang.,...&Lu, Jin-Jian.(2015).Platycodin D triggers autophagy through activation of extracellular signal-regulated kinase in hepatocellular carcinoma HepG2 cells.EUROPEAN JOURNAL OF PHARMACOLOGY,749,81-88. |
| MLA | Li, Ting,et al."Platycodin D triggers autophagy through activation of extracellular signal-regulated kinase in hepatocellular carcinoma HepG2 cells".EUROPEAN JOURNAL OF PHARMACOLOGY 749(2015):81-88. |
入库方式： OAI收割
来源：上海药物研究所
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