A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling
文献类型：期刊论文
| 作者 | Wu, Zhixiang1,2; Cheng, Zhongyi3; Sun, Mingwei1,2; Wan, Xuelian1,2; Liu, Ping1,2; He, Tieming3; Tan, Minjia1,2 ; Zhao, Yingming1,2,4
|
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2015-02 |
| 卷号 | 14期号:2页码:329-339 |
| ISSN号 | 1535-9476 |
| DOI | 10.1074/mcp.M114.044255 |
| 文献子类 | Article |
| 英文摘要 | Methylation of lysine residues on histone proteins is known to play an important role in chromatin structure and function. However, non-histone protein substrates of this modification remain largely unknown. An effective approach for system-wide analysis of protein lysine methylation, particularly lysine monomethylation, is lacking. Here we describe a chemical proteomics approach for global screening for monomethyllysine substrates, involving chemical propionylation of monomethylated lysine, affinity enrichment of the modified monomethylated peptides, and HPLC/MS/MS analysis. Using this approach, we identified with high confidence 446 lysine monomethylation sites in 398 proteins, including three previously unknown histone monomethylation marks, representing the largest data set of protein lysine monomethylation described to date. Our data not only confirms previously discovered lysine methylation substrates in the nucleus and spliceosome, but also reveals new substrates associated with diverse biological processes. This method hence offers a powerful approach for dynamic study of protein lysine monomethylation under diverse cellular conditions and in human diseases. |
| WOS关键词 | MASS-SPECTROMETRY ; POSTTRANSLATIONAL MODIFICATIONS ; PROTEIN METHYLATION ; INTERACTION NETWORKS ; HISTONE METHYLATION ; DRUG DISCOVERY ; IN-VIVO ; ACETYLATION ; METHYLTRANSFERASES ; ENRICHMENT |
| 资助项目 | China Postdoctoral Science Foundation[2013M541567] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2012ZX09301001-007] ; National Basic Research Program of China (973 Program)[2014CBA02004] ; Natural Science Foundation of China[31370814] ; National Science AMP[00000000] ; Technology Major Project 'Key New Drug Creation and Manufacturing Program' of China[2014ZX09507-002] ; Shanghai Pujiang Program[13PJ1410300] ; NIH[GM105933] ; NIH[CA160036] ; NIH[RR020839] ; American Cancer Society[RSG-13-198-01-DDC] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000349144000006 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276657]  |
| 专题 | 化学蛋白质组学研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhao, Yingming |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China; 3.Jingjie PTM BioLab Hangzhou Co Ltd, Hangzhou, Zhejiang, Peoples R China; 4.Univ Chicago, Ben May Dept Canc Res, 924 E 57th St Knapp R120, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Wu, Zhixiang,Cheng, Zhongyi,Sun, Mingwei,et al. A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(2):329-339. |
| APA | Wu, Zhixiang.,Cheng, Zhongyi.,Sun, Mingwei.,Wan, Xuelian.,Liu, Ping.,...&Zhao, Yingming.(2015).A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling.MOLECULAR & CELLULAR PROTEOMICS,14(2),329-339. |
| MLA | Wu, Zhixiang,et al."A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling".MOLECULAR & CELLULAR PROTEOMICS 14.2(2015):329-339. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。